Oligonucleotide primers for construction of SIRP-IgG fusion proteins
SIRPα-D1D2 forward | 5′-TAGTAGAAGCTTATGGAGCCCGCCGGCCCGGCC-3′ |
| SIRPα-D1D2 reverse | 5′-TAGTAGGTCGACAACTCGGATGGTCTCAGACAAGTTGG-3′ |
| SIRPβ1-D1D2 forward | 5′-TAGTAGAAGCTTAGACTCACAGGAGTGGCA-3′ |
| SIRPβ1-D1D2 reverse | 5′-TAGTAGGTCGACTGGAACTCGGATGGCCTCAG-3′ |
| SIRPβ2-D1D2 forward | 5′-TAGTAGAAGCTTCTCCAAAATGCCTGTCCCAGCCTCC-3′ |
| SIRPβ2-D1D2 reverse | 5′-TAGTAGGTCGACGGCCTCAGACAAGTTGGCAGTCCC-3′ |
| Human IgG-Fc forward | 5′-TAGTAGGTCGACAAAACTCACACATGCCC-3′ |
| Human IgG-Fc reverse | 5′-TAGTAGGGATCCTCATTTACCCGGAGACAGGG-3′ |
SIRPα-D1D2 forward | 5′-TAGTAGAAGCTTATGGAGCCCGCCGGCCCGGCC-3′ |
| SIRPα-D1D2 reverse | 5′-TAGTAGGTCGACAACTCGGATGGTCTCAGACAAGTTGG-3′ |
| SIRPβ1-D1D2 forward | 5′-TAGTAGAAGCTTAGACTCACAGGAGTGGCA-3′ |
| SIRPβ1-D1D2 reverse | 5′-TAGTAGGTCGACTGGAACTCGGATGGCCTCAG-3′ |
| SIRPβ2-D1D2 forward | 5′-TAGTAGAAGCTTCTCCAAAATGCCTGTCCCAGCCTCC-3′ |
| SIRPβ2-D1D2 reverse | 5′-TAGTAGGTCGACGGCCTCAGACAAGTTGGCAGTCCC-3′ |
| Human IgG-Fc forward | 5′-TAGTAGGTCGACAAAACTCACACATGCCC-3′ |
| Human IgG-Fc reverse | 5′-TAGTAGGGATCCTCATTTACCCGGAGACAGGG-3′ |
Restriction sites are underlined.