Yield of donor CD8+ T cells in the different tissues of the anti-CD3- or TBI-conditioned NOD recipients 5 days after BMT (n = 6, mean ± SE)
Conditioning and recipient tissues . | Yield of mononuclear cells, ×106 . | % Donor-type CD8+ T cells . | Yield of donor-type CD8+ T cells, ×106 . |
|---|---|---|---|
| Anti-CD3 | |||
| Liver | 3.2 ± 0.5 | 5.6 ± 1.1 | 0.17 ± 0.08 |
| Spleen | 282 ± 23 | 4.7 ± 1.5 | 14.5 ± 1.2 |
| Lymph nodes* | 3.6 ± 0.7 | 7.5 ± 2.2 | 0.31 ± 0.11 |
| TBI | |||
| Liver | 2.1 ± 0.8 | 76 ± 4.5 | 1.86 ± 0.12 |
| Spleen | 12 ± 3.2 | 61 ± 8.9 | 8.3 ± 0.7 |
| Lymph nodes | 0.4 ± 0.1 | 71 ± 5.4 | 0.28 ± 0.17 |
Conditioning and recipient tissues . | Yield of mononuclear cells, ×106 . | % Donor-type CD8+ T cells . | Yield of donor-type CD8+ T cells, ×106 . |
|---|---|---|---|
| Anti-CD3 | |||
| Liver | 3.2 ± 0.5 | 5.6 ± 1.1 | 0.17 ± 0.08 |
| Spleen | 282 ± 23 | 4.7 ± 1.5 | 14.5 ± 1.2 |
| Lymph nodes* | 3.6 ± 0.7 | 7.5 ± 2.2 | 0.31 ± 0.11 |
| TBI | |||
| Liver | 2.1 ± 0.8 | 76 ± 4.5 | 1.86 ± 0.12 |
| Spleen | 12 ± 3.2 | 61 ± 8.9 | 8.3 ± 0.7 |
| Lymph nodes | 0.4 ± 0.1 | 71 ± 5.4 | 0.28 ± 0.17 |
Italics indicate marked difference of yield.
The pooled inguinal and mesenteric lymph nodes