Induction of mixed chimerism in anti-CD3-conditioned recipients given different dose of donor CD8+ T and BM cells
Donor . | Donor CD8+ T dose × 106 . | Donor BM dose, × 106 . | Recipient . | % Chimeric recipients . | % Donor-type cells in PBMNCs . |
|---|---|---|---|---|---|
| FVB/N | 20 × 2 | 200 × 2 | NOD | 90% | 43.7-69.6 |
| FVB/N | 10 × 2 | 200 × 1 | NOD | 100% | 39.7-57.5 |
| FVB/N | 5 × 2 | 100 × 1 | NOD | 63% | 34.8-51.4 |
| C57BL/6 | 10 × 2 | 200 × 1 | BALB/c | 100% | 45.6-71.3 |
| B10A | 10 × 2 | 200 × 1 | C57BL/6 | 100% | 50.1-78.1 |
Donor . | Donor CD8+ T dose × 106 . | Donor BM dose, × 106 . | Recipient . | % Chimeric recipients . | % Donor-type cells in PBMNCs . |
|---|---|---|---|---|---|
| FVB/N | 20 × 2 | 200 × 2 | NOD | 90% | 43.7-69.6 |
| FVB/N | 10 × 2 | 200 × 1 | NOD | 100% | 39.7-57.5 |
| FVB/N | 5 × 2 | 100 × 1 | NOD | 63% | 34.8-51.4 |
| C57BL/6 | 10 × 2 | 200 × 1 | BALB/c | 100% | 45.6-71.3 |
| B10A | 10 × 2 | 200 × 1 | C57BL/6 | 100% | 50.1-78.1 |
PBMNCs indicates peripheral blood mononuclear cells.
× 1 or × 2 indicates 1 or 2 injections.