Parallel increase in the frequencies of CFU-E colonies and the EP phenotype in BM before and after PHZ-induced anemia
. | CFU-E colonies per 105 total BM cells . | . | EP phenotype per 105 total BM cells . | . | ||
|---|---|---|---|---|---|---|
. | PBS control . | PHZ-treated* . | PBS control . | PHZ-treated* . | ||
| Exp. 1 | 245 | 520 | 400 | 912 | ||
| Exp. 2 | 255 | 610 | 378 | 887 | ||
. | CFU-E colonies per 105 total BM cells . | . | EP phenotype per 105 total BM cells . | . | ||
|---|---|---|---|---|---|---|
. | PBS control . | PHZ-treated* . | PBS control . | PHZ-treated* . | ||
| Exp. 1 | 245 | 520 | 400 | 912 | ||
| Exp. 2 | 255 | 610 | 378 | 887 | ||
Frequencies of CFU-E colonies (left) and the EP population (right) were determined in methylcellulose assays and by flow cytometry 3 days after a single injection of PHZ (60 mg/kg body weight). Control mice were injected with PBS only. Both CFU-E colony and EP frequencies increased more than 2-fold.
Measured 3 days after a single injection of PHZ (60 mg/kg body weight).