Table 3.

Release of cytokines by monocytes cultured for 3 or 5 days in the presence of Trx80, GM-CSF + IL-4, or medium alone


Culture conditions

TNFα

IL-1β

IL-6

IL-8

IL-10

IL-12
3 d
5 d
3 d
5 d
3 d
5 d
3 d
5 d
3 d
5 d
3 d
5 d
Medium alone   < 20   < 20   < 20   < 20   < 20   < 20   > 5000   > 5000   < 20   < 20   < 20   < 20  
GM-CSF + IL-4   < 20   < 20   32   27   < 20   < 20   > 5000   > 5000   < 20   < 20   < 20   < 20  
100 nM Trx80
 
166
 
92
 
1621
 
1621
 
> 5000
 
> 5000
 
> 5000
 
> 5000
 
220
 
139
 
< 20
 
< 20
 

Culture conditions

TNFα

IL-1β

IL-6

IL-8

IL-10

IL-12
3 d
5 d
3 d
5 d
3 d
5 d
3 d
5 d
3 d
5 d
3 d
5 d
Medium alone   < 20   < 20   < 20   < 20   < 20   < 20   > 5000   > 5000   < 20   < 20   < 20   < 20  
GM-CSF + IL-4   < 20   < 20   32   27   < 20   < 20   > 5000   > 5000   < 20   < 20   < 20   < 20  
100 nM Trx80
 
166
 
92
 
1621
 
1621
 
> 5000
 
> 5000
 
> 5000
 
> 5000
 
220
 
139
 
< 20
 
< 20
 

Culture media from TAMs generated in the presence of 100 nM Trx80 and from CD14+ monocytes incubated in medium alone (at 106 cells/mL) or in the presence of GM-CSF + IL-4 (4 × 105 cells/mL) were collected after 3 and 5 days. Cytokine concentrations were determined by using the Cytometric Bead Array (CBD) assay and are expressed as picogram per milliliter. The lower limit of detection of this assay was 20 pg/mL. Data from 1 representative experiment of 3 performed with cells from different blood donors are shown.

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