Release of cytokines by monocytes cultured for 3 or 5 days in the presence of Trx80, GM-CSF + IL-4, or medium alone
Culture conditions . | TNFα . | . | IL-1β . | . | IL-6 . | . | IL-8 . | . | IL-10 . | . | IL-12 . | . | ||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
. | 3 d . | 5 d . | 3 d . | 5 d . | 3 d . | 5 d . | 3 d . | 5 d . | 3 d . | 5 d . | 3 d . | 5 d . | ||||||
Medium alone | < 20 | < 20 | < 20 | < 20 | < 20 | < 20 | > 5000 | > 5000 | < 20 | < 20 | < 20 | < 20 | ||||||
GM-CSF + IL-4 | < 20 | < 20 | 32 | 27 | < 20 | < 20 | > 5000 | > 5000 | < 20 | < 20 | < 20 | < 20 | ||||||
100 nM Trx80 | 166 | 92 | 1621 | 1621 | > 5000 | > 5000 | > 5000 | > 5000 | 220 | 139 | < 20 | < 20 |
Culture conditions . | TNFα . | . | IL-1β . | . | IL-6 . | . | IL-8 . | . | IL-10 . | . | IL-12 . | . | ||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
. | 3 d . | 5 d . | 3 d . | 5 d . | 3 d . | 5 d . | 3 d . | 5 d . | 3 d . | 5 d . | 3 d . | 5 d . | ||||||
Medium alone | < 20 | < 20 | < 20 | < 20 | < 20 | < 20 | > 5000 | > 5000 | < 20 | < 20 | < 20 | < 20 | ||||||
GM-CSF + IL-4 | < 20 | < 20 | 32 | 27 | < 20 | < 20 | > 5000 | > 5000 | < 20 | < 20 | < 20 | < 20 | ||||||
100 nM Trx80 | 166 | 92 | 1621 | 1621 | > 5000 | > 5000 | > 5000 | > 5000 | 220 | 139 | < 20 | < 20 |
Culture media from TAMs generated in the presence of 100 nM Trx80 and from CD14+ monocytes incubated in medium alone (at 106 cells/mL) or in the presence of GM-CSF + IL-4 (4 × 105 cells/mL) were collected after 3 and 5 days. Cytokine concentrations were determined by using the Cytometric Bead Array (CBD) assay and are expressed as picogram per milliliter. The lower limit of detection of this assay was 20 pg/mL. Data from 1 representative experiment of 3 performed with cells from different blood donors are shown.