Table 2.

Endocytosis of FITC-labeled dextran particles by TAMs

Period of culture
3 d
5 d
Fluorescent staining for monocytes
% +
MFI
% +
MFI
Control cells in medium alone     
    CD14   50   62   34   58  
    MR   55   59   53   53  
    FITC-Dx at 4°C   3   47   3   50  
    FITC-Dx at 37°C*   42   45   17   57  
TAMs     
    CD14   92   168   77   60  
    MR   96   266   97   317  
    FITC-Dx at 4°C   3   27   3   38  
    FITC-Dx at 37°C*   75   34   68   43  
Stimulated with GM-CSF + IL-4     
    CD14   7   39   1   44  
    MR   91   122   84   126  
    FITC-Dx at 4°C   3   31   3   46  
    FITC-Dx at 37°C*
 		 16
 		 44
 		 20
 		 61
 
Period of culture
3 d
5 d
Fluorescent staining for monocytes
% +
MFI
% +
MFI
Control cells in medium alone     
    CD14   50   62   34   58  
    MR   55   59   53   53  
    FITC-Dx at 4°C   3   47   3   50  
    FITC-Dx at 37°C*   42   45   17   57  
TAMs     
    CD14   92   168   77   60  
    MR   96   266   97   317  
    FITC-Dx at 4°C   3   27   3   38  
    FITC-Dx at 37°C*   75   34   68   43  
Stimulated with GM-CSF + IL-4     
    CD14   7   39   1   44  
    MR   91   122   84   126  
    FITC-Dx at 4°C   3   31   3   46  
    FITC-Dx at 37°C*
 		 16
 		 44
 		 20
 		 61
 

Monocytes were cultured in the absence (control) or presence of GM-CSF + IL-4 or 100 nM Trx80 (TAMs) for 3 or 5 days. After harvesting, washing, and resuspension in 0.5 mL ice-cold PBS to obtain a concentration of 2-6 × 105 cells/mL, 40 μg FITC-conjugated dextran (FITC-Dx) was added, and the cells were incubated for an additional hour at 4°C (as control for nonspecific binding) or 37°C. Finally, the cells were washed and their fluorescence was analyzed by flow cytometry. The values presented are from 1 representative experiment of 3 performed with cells from different blood donors.

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