Table 2. Cytokine secretion and effect... | American Society of Hematology

Table 2.

Cytokine secretion and effect of PGE₂ inhibitor NS-398 on immune cells

	Cytokine secretion
	DCs, TNF-α, pg/mL^*							T cells, IFN-γ, pg/mL^†
	Without MSCs			With MSCs			Without MSCs		With MSCs
Inhibitor	−	+	−	+	−	+	−	+
Experiment no. 1	789 ± 42	880 ± 46	460 ± 44	624 ± 92	1750 ± 32	1600 ± 58	695 ± 100	1390 ± 121
Experiment no. 2	1630 ± 55	1600 ± 57	289 ± 12	1289 ± 57	2287 ± 60	2800 ± 75	1235 ± 80	2764 ± 85
Experiment no. 3	458 ± 30	524 ± 38	144 ± 64	369 ± 48	3196 ± 101	ND	1657 ± 34	2677 ± 74

	Cytokine secretion
	DCs, TNF-α, pg/mL^*							T cells, IFN-γ, pg/mL^†
	Without MSCs			With MSCs			Without MSCs		With MSCs
Inhibitor	−	+	−	+	−	+	−	+
Experiment no. 1	789 ± 42	880 ± 46	460 ± 44	624 ± 92	1750 ± 32	1600 ± 58	695 ± 100	1390 ± 121
Experiment no. 2	1630 ± 55	1600 ± 57	289 ± 12	1289 ± 57	2287 ± 60	2800 ± 75	1235 ± 80	2764 ± 85
Experiment no. 3	458 ± 30	524 ± 38	144 ± 64	369 ± 48	3196 ± 101	ND	1657 ± 34	2677 ± 74

ND indicates not done; −, no inhibitor; +, 5 μM NS-398.

Human MSCs and mature DCs were cocultured with or without PGE₂ inhibitor NS-398 in the presence of activating stimulus LPS. The TNF-α levels in the supernatant were determined by ELISA. Inhibition of PGE₂ always resulted in an increase in TNF-α levels.

†

Human MSCs and T cells were cocultured with or without PGE₂ inhibitor NS-398 in the presence of mitogen PHA and levels of IFN-γ were determined by ELISA. Results show that inhibition of PGE₂ always resulted in an increase in IFN-γ levels.

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