Inhibition of PHA-stimulated PBMC proliferation by human MSCs
. | PBMC no. 1001 . | . | PBMC no. 1002 . | . | PBMC no. 11-30338 . | . | |||
|---|---|---|---|---|---|---|---|---|---|
| No. MSCs . | PBMCs + PHA . | PBMCs + PHA + MSCs . | PBMCs + PHA . | PBMCs + PHA + MSCs . | PBMCs + PHA . | PBMCs + PHA + MSCs . | |||
| 51 | 25 044 ± 2849 | 9 717 ± 1379 | 12 188 ± 2105 | 5564 ± 594 | 34 799 ± 3312 | 10 474 ± 3954 | |||
| 57 | 25 044 ± 2849 | 12 727 ± 958 | 12 188 ± 2105 | 5434 ± 4039 | 34 799 ± 3312 | 15 159 ± 3359 | |||
| 78 | 25 044 ± 2849 | 11 236 ± 1250 | 12 188 ± 2105 | 7458 ± 973 | 34 799 ± 3312 | 11 649 ± 2319 | |||
| 101 | 25 044 ± 2849 | 12 070 ± 2207 | 12 188 ± 2105 | 6841 ± 3334 | 34 799 ± 3312 | 10 556 ± 4461 | |||
| 151 | 25 044 ± 2849 | 8 519 ± 1359 | 12 188 ± 2105 | 4172 ± 582 | 34 799 ± 3312 | 9521 ± 597 | |||
. | PBMC no. 1001 . | . | PBMC no. 1002 . | . | PBMC no. 11-30338 . | . | |||
|---|---|---|---|---|---|---|---|---|---|
| No. MSCs . | PBMCs + PHA . | PBMCs + PHA + MSCs . | PBMCs + PHA . | PBMCs + PHA + MSCs . | PBMCs + PHA . | PBMCs + PHA + MSCs . | |||
| 51 | 25 044 ± 2849 | 9 717 ± 1379 | 12 188 ± 2105 | 5564 ± 594 | 34 799 ± 3312 | 10 474 ± 3954 | |||
| 57 | 25 044 ± 2849 | 12 727 ± 958 | 12 188 ± 2105 | 5434 ± 4039 | 34 799 ± 3312 | 15 159 ± 3359 | |||
| 78 | 25 044 ± 2849 | 11 236 ± 1250 | 12 188 ± 2105 | 7458 ± 973 | 34 799 ± 3312 | 11 649 ± 2319 | |||
| 101 | 25 044 ± 2849 | 12 070 ± 2207 | 12 188 ± 2105 | 6841 ± 3334 | 34 799 ± 3312 | 10 556 ± 4461 | |||
| 151 | 25 044 ± 2849 | 8 519 ± 1359 | 12 188 ± 2105 | 4172 ± 582 | 34 799 ± 3312 | 9521 ± 597 | |||
Human MSCs from 5 different donors were plated onto a 96-well plate (2 × 104 cells/mL) in a 100-μL volume. Following adherence of MSCs, PBMCs from an unrelated donor were added in the presence of PHA (hMSC to PBMC ratio, 1:10). 3H-thymidine incorporation was measured at 72 hours of coculture (set in triplicates) and data were expressed as mean cpm ± SD. The experiment was performed with 2 additional hPBMC donors. The cpm for MSC alone or hPBMC alone was less than 1500 in all experiments.