Antibodies used
Antigen . | Label . | Isotype . | Concentration, μg/mL . | Dilution . | Clone . | Company . |
|---|---|---|---|---|---|---|
| Isotype | FITC | IgM,k | 30 | 1/3 | DAK-GO8 | DAKO |
| Isotype, KLH | — | IgM,k | 500 | 1/200 | C48-6 | BD |
| Heparan sulfate, 10E4 | FITC | IgM,k | 250 | 1/25 | F58-10E4 | Seikaguku |
| Heparan sulfate, 10E4 | — | IgM,k | 500 | 1/200 | 8.S.087 | US Biological |
| Mouse IgG + IgM | FITC | Polyclonal | 500 | 1/25 | Polyclonal | BD |
| CD4 | FITC | IgG1,k | NA | 1/50 | RPA-T4 | BD |
| CD8 | FITC | IgG1,k | NA | 1/50 | SK1 | BD |
| CD14 | FITC | IgG2a,k | NA | 1/50 | M5E2 | BD |
| CD16 | FITC | IgG1 | 40 | 1/50 | 3G8 | Immunotech |
| CD19 | FITC | IgG1,k | 100 | 1/50 | HD37 | DAKO |
| Mouse IgG | Alexa488 | Polyclonal | 2000 | 1/500 | Polyclonal | Molecular Probes |
| LAMP-1 (CD107a) | — | IgG1,k | 100 | 1/2000 | H4A3 | BD |
Antigen . | Label . | Isotype . | Concentration, μg/mL . | Dilution . | Clone . | Company . |
|---|---|---|---|---|---|---|
| Isotype | FITC | IgM,k | 30 | 1/3 | DAK-GO8 | DAKO |
| Isotype, KLH | — | IgM,k | 500 | 1/200 | C48-6 | BD |
| Heparan sulfate, 10E4 | FITC | IgM,k | 250 | 1/25 | F58-10E4 | Seikaguku |
| Heparan sulfate, 10E4 | — | IgM,k | 500 | 1/200 | 8.S.087 | US Biological |
| Mouse IgG + IgM | FITC | Polyclonal | 500 | 1/25 | Polyclonal | BD |
| CD4 | FITC | IgG1,k | NA | 1/50 | RPA-T4 | BD |
| CD8 | FITC | IgG1,k | NA | 1/50 | SK1 | BD |
| CD14 | FITC | IgG2a,k | NA | 1/50 | M5E2 | BD |
| CD16 | FITC | IgG1 | 40 | 1/50 | 3G8 | Immunotech |
| CD19 | FITC | IgG1,k | 100 | 1/50 | HD37 | DAKO |
| Mouse IgG | Alexa488 | Polyclonal | 2000 | 1/500 | Polyclonal | Molecular Probes |
| LAMP-1 (CD107a) | — | IgG1,k | 100 | 1/2000 | H4A3 | BD |
KLH indicates keyhole limpet hemocyanin protein; IgG, immunoglobulin G; IgM, immunoglobulin M; LAMP-1, lysosome-associated membrane protein 1; —, unlabelled; and NA, not available.