Interassay variability of all assays used in this study
Assay . | Mean . | Standard deviation . | Coefficient of variation, % . |
|---|---|---|---|
| dRVVT, coagulation time, s | 66.1 | 2.1 | 3.2 |
| PTT-LA, coagulation time, s | 55.1 | 1.1 | 2.0 |
| β2GPI-dependent LAC, difference in ratio between 25 μM CL and 0 μM CL* | 0.28 | 0.03 | 10.7 |
| aCL ELISA, GPL units | 40.9 | 2.8 | 6.9 |
| Anti-β2GPI IgG ELISA, OD | 0.220 | 0.025 | 11.5 |
| Anti-domain I ELISA, OD | 0.315 | 0.012 | 3.8 |
Assay . | Mean . | Standard deviation . | Coefficient of variation, % . |
|---|---|---|---|
| dRVVT, coagulation time, s | 66.1 | 2.1 | 3.2 |
| PTT-LA, coagulation time, s | 55.1 | 1.1 | 2.0 |
| β2GPI-dependent LAC, difference in ratio between 25 μM CL and 0 μM CL* | 0.28 | 0.03 | 10.7 |
| aCL ELISA, GPL units | 40.9 | 2.8 | 6.9 |
| Anti-β2GPI IgG ELISA, OD | 0.220 | 0.025 | 11.5 |
| Anti-domain I ELISA, OD | 0.315 | 0.012 | 3.8 |
To ensure the quality of the assays used in this study, we determined the interassay variability. For all assays, we performed 10 runs on duplicate samples on different days. The interassay coefficient of variation (CV) is then calculated as follows: (standard deviation of the means of all 10 duplicates/mean of all 10 duplicates) × 100 = % CV.
See Table 1.