Clonogenic assay for cord blood samples in the presence of BIBR1532
. | BFU-E . | CFU-GM . | Total count . |
|---|---|---|---|
| Negative control | 128 ± 111 | 13 ± 8 | 138 ± 117 |
| DMSO | 126 ± 82 | 24 ± 14 | 148 ± 90 |
| 10 μM | 114 ± 98 | 12 ± 8 | 125 ± 105 |
| 30 μM | 133 ± 78 | 24 ± 13 | 155 ± 86 |
| 50 μM | 120 ± 67 | 25 ± 13 | 144 ± 75 |
| 80 μM | 132 ± 82 | 22 ± 11 | 152 ± 89 |
. | BFU-E . | CFU-GM . | Total count . |
|---|---|---|---|
| Negative control | 128 ± 111 | 13 ± 8 | 138 ± 117 |
| DMSO | 126 ± 82 | 24 ± 14 | 148 ± 90 |
| 10 μM | 114 ± 98 | 12 ± 8 | 125 ± 105 |
| 30 μM | 133 ± 78 | 24 ± 13 | 155 ± 86 |
| 50 μM | 120 ± 67 | 25 ± 13 | 144 ± 75 |
| 80 μM | 132 ± 82 | 22 ± 11 | 152 ± 89 |
CD34+ cord blood cells (n = 7) were harvested from suspension culture by day 10, and cultured in serum-free methylcellulose medium supplemented with cytokines (SCF, GM-CSF, G-CSF, IL-3, IL-6, and erythropoietin [EPO]) and with increasing concentrations of BIBR1532 for 14 days. Experiments for each individual specimen were performed in triplicate.
Note: There is no significant difference observed in number or type of colonies at day 14 for those cultured with up to 80 μM BIBR1532 compared with the controls.
BFU-E indicates burst-forming unit erythroid; CFU-GM, colony-forming unit granulocyte/monocyte.