Complementary oligonucleotides used to create single-site reporter constructs
PGL2 promoter constructs . | Annealed oligonucleotides . |
|---|---|
| VWF E4BP4 (Neg 5) | 5′ GATC GGGCTACATAAC |
| CCCGATGTATTG GATC 5′ | |
| Mutant VWF E4BP4 (Neg 5 Mut) | 5′ GATC GGGCTACACTGCAGCA |
| CCCGATGTGACGTCGT GATC 5′ | |
| E4BP4 consensus | 5′ GATC GTTATGTAACG |
| CAATACATTGC GATC 5′ |
PGL2 promoter constructs . | Annealed oligonucleotides . |
|---|---|
| VWF E4BP4 (Neg 5) | 5′ GATC GGGCTACATAAC |
| CCCGATGTATTG GATC 5′ | |
| Mutant VWF E4BP4 (Neg 5 Mut) | 5′ GATC GGGCTACACTGCAGCA |
| CCCGATGTGACGTCGT GATC 5′ | |
| E4BP4 consensus | 5′ GATC GTTATGTAACG |
| CAATACATTGC GATC 5′ |