Real-time RT-PCR for detection of fibromodulin-specific mRNA
Sample . | Product at Tm = 86°C . | Cycles . |
|---|---|---|
| CLL-1 | + | 29.2 |
| CD40L—CLL-1 | + | 30.1 |
| CLL-2 | + | 24.7 |
| CLL-3 | + | 34.7 |
| CLL-4 | + | 31.0 |
| CD40L—CLL-4 | + | 29.6 |
| CLL-5 | + | 26.6 |
| CLL-6 | + | 30.3 |
| CLL-9 | + | 31.7 |
| CD40L—CLL-9 | + | 30.9 |
| D-1 | - | 44.3 |
| D-2 | - | 47.0 |
| D-3 | - | 42.0 |
| T-1 | - | 47.0 |
| Mec-1 | + | 28.3 |
Sample . | Product at Tm = 86°C . | Cycles . |
|---|---|---|
| CLL-1 | + | 29.2 |
| CD40L—CLL-1 | + | 30.1 |
| CLL-2 | + | 24.7 |
| CLL-3 | + | 34.7 |
| CLL-4 | + | 31.0 |
| CD40L—CLL-4 | + | 29.6 |
| CLL-5 | + | 26.6 |
| CLL-6 | + | 30.3 |
| CLL-9 | + | 31.7 |
| CD40L—CLL-9 | + | 30.9 |
| D-1 | - | 44.3 |
| D-2 | - | 47.0 |
| D-3 | - | 42.0 |
| T-1 | - | 47.0 |
| Mec-1 | + | 28.3 |
Given are the numbers of cycles needed for detection of fibromodulin-specific mRNA of unstimulated CLL samples, CD40L-stimulated CLL samples (CD40L-CLL), PBMCs of healthy donors (D), tonsillar B cells (T), and Mec-1 cells.