Cytokine-induced differentiation of HOXB6-immortalized myeloid cell lines
. | Morphology, no. . | . | . | . | . | . | . | . | . | ||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| . | GM-CSF . | . | . | G-CSF . | . | . | M-CSF . | . | . | ||||||||
| Cell line . | Blast . | Neut . | Mono . | Blast . | Neut . | Mono . | Blast . | Neut . | Mono . | ||||||||
| B6 2.0 | 75 | 12 | 13 | 6 | 86 | 8 | 6 | 6 | 87 | ||||||||
| B6 3.0 | 92 | 1 | 7 | 2 | 86 | 12 | 26 | 4 | 70 | ||||||||
| B6WG 2.0 | 83 | 11 | 6 | 5 | 94 | 1 | 83 | 2 | 15 | ||||||||
| B6WG 4.0 | 97 | 1 | 2 | 18 | 73 | 9 | 2 | 1 | 97 | ||||||||
. | Morphology, no. . | . | . | . | . | . | . | . | . | ||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| . | GM-CSF . | . | . | G-CSF . | . | . | M-CSF . | . | . | ||||||||
| Cell line . | Blast . | Neut . | Mono . | Blast . | Neut . | Mono . | Blast . | Neut . | Mono . | ||||||||
| B6 2.0 | 75 | 12 | 13 | 6 | 86 | 8 | 6 | 6 | 87 | ||||||||
| B6 3.0 | 92 | 1 | 7 | 2 | 86 | 12 | 26 | 4 | 70 | ||||||||
| B6WG 2.0 | 83 | 11 | 6 | 5 | 94 | 1 | 83 | 2 | 15 | ||||||||
| B6WG 4.0 | 97 | 1 | 2 | 18 | 73 | 9 | 2 | 1 | 97 | ||||||||
Three hundred cell differentials were performed on Wright-Giemsa-stained cytospins from 2 independently derived HOXB6 and HOXB6WG cell lines grown in GM-CSF, G-CSF, or M-CSF as the sole cytokine.
Neut indicates neutrophils; and Mono, monocytes.