Summary of relative levels of mRNA for nucleoside transport and metabolism genes
. | . | Gene expression . | . | . | ||
|---|---|---|---|---|---|---|
| mRNA . | No. of patients . | Low . | High . | Median . | ||
| hENT1 | 44 | 0.05 | 1.97 | 0.40 | ||
| hENT2 | 45 | 0.24 | 837 | 1.15 | ||
| hCNT3 | 45 | 0.01 | 2.41 | 0.12 | ||
| dCK | 45 | 0.86 | 161 | 4.47 | ||
| CD73 | 45 | 0.00 | 39.7 | 0.43 | ||
| CN-II | 43 | 0.00 | 12.8 | 1.59 | ||
| dNT-1 | 45 | 0.00 | 105 | 1.65 | ||
. | . | Gene expression . | . | . | ||
|---|---|---|---|---|---|---|
| mRNA . | No. of patients . | Low . | High . | Median . | ||
| hENT1 | 44 | 0.05 | 1.97 | 0.40 | ||
| hENT2 | 45 | 0.24 | 837 | 1.15 | ||
| hCNT3 | 45 | 0.01 | 2.41 | 0.12 | ||
| dCK | 45 | 0.86 | 161 | 4.47 | ||
| CD73 | 45 | 0.00 | 39.7 | 0.43 | ||
| CN-II | 43 | 0.00 | 12.8 | 1.59 | ||
| dNT-1 | 45 | 0.00 | 105 | 1.65 | ||
The CT value obtained for a sample was corrected for RNA concentration by subtracting the CT value for GAPDH (ΔCT). The results were then normalized to the expression level in reference peripheral blood mononuclear cells derived from a single healthy donor (reference ΔCT – test ΔCT = ΔΔCT) and expressed as 2–(ΔΔCT) (arbitrary quantitative PCR units) as previously described.15,20,21