Effect of RA on the expression of t-PA, RARα, RARβ, RARγ, and Sp1 mRNA over time
. | Treatment-to-control ratios, mean (SEM) . | . | . | . | . | . | ||||
|---|---|---|---|---|---|---|---|---|---|---|
. | 0 h . | 2 h . | 4 h . | 6 h . | 8 h . | ANOVA . | ||||
| t-PA | 1.0 | 1.3 (0.08) | 1.6 (0.22)* | 1.8 (0.23)† | 2.0 (0.14)† | < .01 | ||||
| RARα | 1.0 | 1.2 (0.07)* | 0.9 (0.11) | 0.9 (0.05) | 0.8 (0.03) | < .01 | ||||
| RARβ | 1.0 | 4.1 (1.68)* | 4.7 (1.28)† | 5.5 (1.58)† | 5.6 (1.27)† | < .01 | ||||
| RARγ | 1.0 | 1.2 (0.07) | 1.6 (0.13)‡ | 1.4 (0.03)† | 1.4 (0.06)† | < .01 | ||||
| Sp1 | 1.0 | 1.0 (0.09) | 1.0 (0.02) | 1.1 (0.03) | 1.0 (0.01) | NS | ||||
. | Treatment-to-control ratios, mean (SEM) . | . | . | . | . | . | ||||
|---|---|---|---|---|---|---|---|---|---|---|
. | 0 h . | 2 h . | 4 h . | 6 h . | 8 h . | ANOVA . | ||||
| t-PA | 1.0 | 1.3 (0.08) | 1.6 (0.22)* | 1.8 (0.23)† | 2.0 (0.14)† | < .01 | ||||
| RARα | 1.0 | 1.2 (0.07)* | 0.9 (0.11) | 0.9 (0.05) | 0.8 (0.03) | < .01 | ||||
| RARβ | 1.0 | 4.1 (1.68)* | 4.7 (1.28)† | 5.5 (1.58)† | 5.6 (1.27)† | < .01 | ||||
| RARγ | 1.0 | 1.2 (0.07) | 1.6 (0.13)‡ | 1.4 (0.03)† | 1.4 (0.06)† | < .01 | ||||
| Sp1 | 1.0 | 1.0 (0.09) | 1.0 (0.02) | 1.1 (0.03) | 1.0 (0.01) | NS | ||||
n = 3. Confluent HUVEC cultures in the second passage were incubated for the indicated time periods with 10-6 M RA or DMSO vehicle (0.01%). Total cellular RNA was extracted, and mRNA was converted into cDNA. Target mRNA (cDNA) was quantified by real-time PCR and normalized relative to glyceraldehyde 3-phosphatedehydrogenase (GAPDH) mRNA. The results are expressed as fold induction compared with control cultures. Each data point represents the average of duplicate wells from 3 different HUVEC cultures, each assayed in triplicate for both target and GAPDH. Post hoc analysis was performed by contrast analysis. NS indicates not significant.
P < .05.
P < .01.
P < .001.