Sequences of oligonucleotides used in the electrophoretic mobility shift assay (only upper strand shown)
Name . | Location . | Sequence . |
|---|---|---|
| Wild-type GC-box | -7363/C/-7339 | CCCAAAGGAGCCCGCCCCAGACACA |
| Mutant GC-box | -7363/T/-7339 | CCCAAAGGAGCCTGCCCCAGACACA |
| Scrambled GC-box | -7363/scrambled/-7339 | CGCAGACACCAGCCACGACGACCAC |
| DR5 | -7323/-7299 | TCTGGGGTCACCCTGGGGTCAGAAG |
| Scrambled DR5 | -7323/scrambled/-7299 | GCAGCTGAGTCGTAGCGTCGAGCTG |
| GC-box/DR5 | -7363/C/-7289 | CCCAAAGGAGCCCGCCCCAGACACA |
| GCCATGGCCTGGGACTCTGGGGTCA | ||
| CCCTGGGGTCAGAAGGAATTATCTG |
Name . | Location . | Sequence . |
|---|---|---|
| Wild-type GC-box | -7363/C/-7339 | CCCAAAGGAGCCCGCCCCAGACACA |
| Mutant GC-box | -7363/T/-7339 | CCCAAAGGAGCCTGCCCCAGACACA |
| Scrambled GC-box | -7363/scrambled/-7339 | CGCAGACACCAGCCACGACGACCAC |
| DR5 | -7323/-7299 | TCTGGGGTCACCCTGGGGTCAGAAG |
| Scrambled DR5 | -7323/scrambled/-7299 | GCAGCTGAGTCGTAGCGTCGAGCTG |
| GC-box/DR5 | -7363/C/-7289 | CCCAAAGGAGCCCGCCCCAGACACA |
| GCCATGGCCTGGGACTCTGGGGTCA | ||
| CCCTGGGGTCAGAAGGAATTATCTG |
The polymorphic nucleotide is indicated in bold type, and the GC-box and DR5 (RA receptor responsive element [RARE], spaced by a 5 base sequence) sequences are underlined.