The distribution of cells within G1, S, and G2 phases of the cell cycle in CEM, HEL-92, and HL60 cells
. | CEM, mean % ± SD . | . | . | HEL-92, mean % ± SD . | . | . | HL60, mean % ± SD . | . | . | ||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
. | G1 . | S . | G2 . | G1 . | S . | G2 . | G1 . | S . | G2 . | ||||||
| Control | 37 ± 2 | 28 ± 1 | 35 ± 3 | 58 ± 4 | 13 ± 1 | 28 ± 3 | 49 ± 5 | 12 ± 3 | 39 ± 2 | ||||||
| 6 h | 38 ± 3 | 26 ± 1 | 36 ± 2 | 56 ± 5 | 14 ± 2 | 30 ± 3 | 47 ± 4 | 13 ± 1 | 40 ± 4 | ||||||
| 2 d | 34 ± 1 | 28 ± 2 | 38 ± 3 | 55 ± 3 | 14 ± 2 | 31 ± 4 | 50 ± 6 | 12 ± 2 | 38 ± 5 | ||||||
. | CEM, mean % ± SD . | . | . | HEL-92, mean % ± SD . | . | . | HL60, mean % ± SD . | . | . | ||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
. | G1 . | S . | G2 . | G1 . | S . | G2 . | G1 . | S . | G2 . | ||||||
| Control | 37 ± 2 | 28 ± 1 | 35 ± 3 | 58 ± 4 | 13 ± 1 | 28 ± 3 | 49 ± 5 | 12 ± 3 | 39 ± 2 | ||||||
| 6 h | 38 ± 3 | 26 ± 1 | 36 ± 2 | 56 ± 5 | 14 ± 2 | 30 ± 3 | 47 ± 4 | 13 ± 1 | 40 ± 4 | ||||||
| 2 d | 34 ± 1 | 28 ± 2 | 38 ± 3 | 55 ± 3 | 14 ± 2 | 31 ± 4 | 50 ± 6 | 12 ± 2 | 38 ± 5 | ||||||
Cells were cultured with 60 μM THC (cytotoxic concentration), and cell cycle distribution was assessed by flow cytometry. The sub-G1 population of cells (apoptotic) was excluded from these analyses. Data points are the mean percentages and SDs of 3 separate experiments.