Expression of CXCR1 and CXCR2 IL-8 receptors on CD34+ cells
. | CXCR1 . | . | CXCR2 . | . | ||
|---|---|---|---|---|---|---|
. | % . | MFI . | % . | MFI . | ||
| NPB1 | 97 | 18 | 30 | 8 | ||
| NPB2 | 70 | 8 | 18 | 7 | ||
| NPB3 | 60 | 10 | Ud | Ud | ||
| NPB4 | 89 | 11 | Ud | Ud | ||
| NPB5 | 94 | 13 | 25 | 6 | ||
| MMM1 | ||||||
| Population 1 | 39 | 10 | 71 | 10 | ||
| Population 2 | Ud | Ud | 8 | 183 | ||
| MMM2 | ND | ND | ND | ND | ||
| MMM3 | 90 | 32 | 96 | 39 | ||
| MMM4 | ND | ND | 73 | 19 | ||
| MMM5 | 97 | 14 | 95 | 10 | ||
| MMM6 | ND | ND | ND | ND | ||
| MMM7 | 90 | 21 | 4 | 14 | ||
| MMM8 | 99 | 20 | 77 | 7 | ||
| MMM9 | 7 | 18 | 20 | 41 | ||
| MMM10 | 88 | 15 | 97 | 16 | ||
. | CXCR1 . | . | CXCR2 . | . | ||
|---|---|---|---|---|---|---|
. | % . | MFI . | % . | MFI . | ||
| NPB1 | 97 | 18 | 30 | 8 | ||
| NPB2 | 70 | 8 | 18 | 7 | ||
| NPB3 | 60 | 10 | Ud | Ud | ||
| NPB4 | 89 | 11 | Ud | Ud | ||
| NPB5 | 94 | 13 | 25 | 6 | ||
| MMM1 | ||||||
| Population 1 | 39 | 10 | 71 | 10 | ||
| Population 2 | Ud | Ud | 8 | 183 | ||
| MMM2 | ND | ND | ND | ND | ||
| MMM3 | 90 | 32 | 96 | 39 | ||
| MMM4 | ND | ND | 73 | 19 | ||
| MMM5 | 97 | 14 | 95 | 10 | ||
| MMM6 | ND | ND | ND | ND | ||
| MMM7 | 90 | 21 | 4 | 14 | ||
| MMM8 | 99 | 20 | 77 | 7 | ||
| MMM9 | 7 | 18 | 20 | 41 | ||
| MMM10 | 88 | 15 | 97 | 16 | ||
CD34+ cells purified from either MMM or NPB were labeled with PE-conjugated anti-CXCR1 and anti-CXCR2 or isotype control. The percentage and MFI of positive cells were analyzed on 5 × 104 cells with FACScalibur flow cytometer. Note that patient MMM1 exhibited 2 distinct CXCR2-expressing populations.
Ud indicates undetectable; ND, not determined.