Inhibition of IL-8 production, in CD34+-derived cell culture, by either IL-8 antisense or anti-IL-8 antibody
. | IL-8 production . | . | . | ||
|---|---|---|---|---|---|
. | Controls, pg/mL* . | With IL-8 antisense or antibody, pg/mL . | Inhibition, % . | ||
| Cultured with or without 5 μM IL-8 antisense | |||||
| H1 | 11 460 | 3270 | 71 | ||
| H2 | 1 800 | 1120 | 40 | ||
| H3 | 2 475 | 1284 | 50 | ||
| MMM3 | 4 652 | 1260 | 73 | ||
| MMM8 | 9 760 | 2280 | 77 | ||
| MMM9 | 3 176 | 2140 | 33 | ||
| MMM10 | 3 850 | 2850 | 25 | ||
| Cultured with or without 10 μg/mL anti-IL-8 antibody | |||||
| H1 | 11 680 | 592 | 94 | ||
| H2 | 12 786 | 4944 | 61 | ||
| H3 | 2 880 | 60 | 97 | ||
| H4 | 4 060 | < 8 | 100 | ||
| MMM1 | 2 995 | < 8 | 100 | ||
| MMM2 | 2 600 | < 8 | 100 | ||
| MMM3 | 4 020 | 120 | 97 | ||
| MMM4 | 10 080 | 180 | 98 | ||
| MMM5 | 5 740 | 930 | 83 | ||
| MMM6 | 3 950 | 3500 | 11 | ||
| MMM7 | 2 478 | 2000 | 19 | ||
. | IL-8 production . | . | . | ||
|---|---|---|---|---|---|
. | Controls, pg/mL* . | With IL-8 antisense or antibody, pg/mL . | Inhibition, % . | ||
| Cultured with or without 5 μM IL-8 antisense | |||||
| H1 | 11 460 | 3270 | 71 | ||
| H2 | 1 800 | 1120 | 40 | ||
| H3 | 2 475 | 1284 | 50 | ||
| MMM3 | 4 652 | 1260 | 73 | ||
| MMM8 | 9 760 | 2280 | 77 | ||
| MMM9 | 3 176 | 2140 | 33 | ||
| MMM10 | 3 850 | 2850 | 25 | ||
| Cultured with or without 10 μg/mL anti-IL-8 antibody | |||||
| H1 | 11 680 | 592 | 94 | ||
| H2 | 12 786 | 4944 | 61 | ||
| H3 | 2 880 | 60 | 97 | ||
| H4 | 4 060 | < 8 | 100 | ||
| MMM1 | 2 995 | < 8 | 100 | ||
| MMM2 | 2 600 | < 8 | 100 | ||
| MMM3 | 4 020 | 120 | 97 | ||
| MMM4 | 10 080 | 180 | 98 | ||
| MMM5 | 5 740 | 930 | 83 | ||
| MMM6 | 3 950 | 3500 | 11 | ||
| MMM7 | 2 478 | 2000 | 19 | ||
See Table 1 footnote for abbreviations.
CD34+ cells purified from the peripheral blood of either patients with MMM or healthy subjects were cultured in serum-free medium containing cytokines in the presence and absence of IL-8 antisense or antihuman IL-8 antibody. After 14 days of culture, supernatants were harvested, and the IL-8 level was measured by ELISA. The threshold sensitivity for the IL-8 ELISA was ≥ 8 pg/mL.
Matched random controls for IL-8 antisense; isotypic controls for anti—IL-8 antibody.