Total BFU-Es and CFU-Es in wild-type and STAT1–/– mice
Source . | Wild-type . | STAT1-/- . |
|---|---|---|
| Bone marrow | ||
| Cells, × 108/mouse | 1.46 ± 0.05 | 0.95 ± 0.04* |
| BFU-E, × 104/mouse | 9.51 ± 0.48 | 9.95 ± 1.94 |
| CFU-E, × 105/mouse | 12.86 ± 0.63 | 7.76 ± 0.87* |
| Spleen | ||
| Cells, × 108/spleen | 0.85 ± 0.15 | 0.93 ± 0.08 |
| BFU-E, × 103/spleen | 8.15 ± 2.20 | 18.0 ± 2.20† |
| CFU-E, × 104/spleen | 0.83 ± 0.61 | 3.77 ± 0.53† |
| Total body | ||
| BFU-E | 1.03 × 105 | 1.18 × 105 |
| CFU-E | 1.29 × 106 | 8.14 × 105* |
Source . | Wild-type . | STAT1-/- . |
|---|---|---|
| Bone marrow | ||
| Cells, × 108/mouse | 1.46 ± 0.05 | 0.95 ± 0.04* |
| BFU-E, × 104/mouse | 9.51 ± 0.48 | 9.95 ± 1.94 |
| CFU-E, × 105/mouse | 12.86 ± 0.63 | 7.76 ± 0.87* |
| Spleen | ||
| Cells, × 108/spleen | 0.85 ± 0.15 | 0.93 ± 0.08 |
| BFU-E, × 103/spleen | 8.15 ± 2.20 | 18.0 ± 2.20† |
| CFU-E, × 104/spleen | 0.83 ± 0.61 | 3.77 ± 0.53† |
| Total body | ||
| BFU-E | 1.03 × 105 | 1.18 × 105 |
| CFU-E | 1.29 × 106 | 8.14 × 105* |
Single-cell suspensions were isolated from the bone marrow (both femurs) and spleen of individual mice using a 70-μm cell strainer. Erythrocytes were lysed in 2% acetic acid and nucleated cells were counted with the use of a hemacytometer. Shown is the mean ± SE bone marrow and spleen cellularity from analysis of 3 wild-type and 3 STAT1-/- mice. Numbers of BFU-Es and CFU-Es per bone marrow and spleen were obtained using the data from Figures 1 and 2 at 1 U/mL EPO. The data are the mean ± SE for 3 animals (*P < .01; †P < .05).