Table 4. Junctional sequences of the... | American Society of Hematology

Table 4.

Junctional sequences of the clonal TCR-β and TCR-γ rearrangements

Case	3′V sequence	N-(D)-N sequence	5′J sequence	V (D) J sequence
5	TGTGCCACCTGGGACGG	TATAGTAGTGATTGGC	TCAAGACGTTT	Vγ2.1 JPγ2.1
7	TCAAGACGTTT	CC	TTATTATAAGAAACTCTTT	Vγ4.2 Jγ2
11	TTCTACATCTGCAGTGCTAG	GCCAGGACAATGCGACCGC	GAGACCCAGTACTTCGGG	Vβ29.1 Dβ1 Jβ2.5
12 S	TGTGCCACCTGGGATA	A	AAACTCTTT	Vγ8.1 Jγ2.1
12 LN	TGTGCCACCTGGGATA	A	AAACTCTTT	Vγ8.1 Jγ2.1
15	TGCGCCAGCAGT	TTACTAAAACCAC	CTAACTATGGCTACACC	Vβ10.1 Dβ 2 Jβ1.2
16	TTGATTTACTTC	CAGGGTAACAGT	CAACTAGACAAA	Vβ7.2 Dβ2 Jβ1.3
17	TGTGCCAGTCA	TAAAGGGAGGGT	CTGATCTCTACG	Vβ30.4 Dβ2 Jβ2.1
18	CAGTA	TTAGGCTAGCGGG	CCTACGACAGTACTTCGG	Vβ29.1 Dβ2 Jβ2.7
19	TGTGCCAGTAGT	TCGGC	AGCAATCAGCCCCAGCATT	Vβ7.2 Dβ1 Jβ1.5
20	TGTGCCACCTGGGATGGG	C	TATAAGAAACTCTTT	Vγ4.2 Jγ2.1
23	TGTGCCAGCAGC	CCGCTACAGGGA	TCCTACGAGCAGTACTTCG	Vβ7.9 Dβ2 Jβ2.7
25*	TGTGATCTTGCTGAAGGAAGTA		GACTAAGCTCATAGTAACT	Vγ4.1 JPγ1.1
25*	TGTGCCACCTGGGATAGG		ATTATTATAAGAAACTCTTT	Vγ8.1 Jγ2.1
26 S	TGTGCCAGCAGC	TGGGACAGGGGTAG	CTCCTACGAGCAGTACCAA	Vβ5.4 Dβ1 Jβ2.7
26 LN	TGTGCCAGCAGC	TGGGACAGGGGTAG	CTCCTACGAGCAGTACCAA	Vβ5.4 Dβ1 Jβ2.7
27	TGTGATCTTGCTGAAGGAAGTA		ATTATTATAAGAAACTCT	Vγ4.1 J γ2.1
28	CAGCTT	CGACAGGGC	CAGATACGCAGTATTTTGG	Vβ13.2 Dβ1 Jβ2.3
30	TTCTACATCTGCAGTGCTAGAG	CTACGGCGGTAGCCTAT	GAGACCCAGTACTCGGGC	Vβ30.5 Dβ2 Jβ2.5
31	AGTGCTGACTAGCGG	GGCTGGGGG	AGACCCAGTACTTCC	Vβ20.1 Dβ2 Jβ2-5
32	AGCACC	AGTATCGCTAGCTCA	ACAATGAGCAGTTCTTCGG	Vβ30.4 Dβ2 Jβ2.1
33 S	TGTGCCACCTGGGATA	AGGAC	TATTATAAGAAACTCTTT	V γ8.1 J γ2.1
33 LN	TGTGCCACCTGGGA	AGGAC	TATTATAAGAAACTCTTT	Vγ8.1 J γ2.1
35	TGTGCCACC	CC	TTATTATAAGAAACTCTTT	V γ4.1 J γ2.1
MOLT†	TTATTATAAGAAACTCTTT	AGAGTCGACTAGCGATCCAAAA	AATGAGCAGTTCTTCGGG	Vβ20.1 Dβ2 J β2.1
HUT†	TCTGCAGCGT	AGGCAGGAGGCGGTTGGC	GGCTACACCTTCGGTTC	Vβ30 Dβ2 J β1.2

Case	3′V sequence	N-(D)-N sequence	5′J sequence	V (D) J sequence
5	TGTGCCACCTGGGACGG	TATAGTAGTGATTGGC	TCAAGACGTTT	Vγ2.1 JPγ2.1
7	TCAAGACGTTT	CC	TTATTATAAGAAACTCTTT	Vγ4.2 Jγ2
11	TTCTACATCTGCAGTGCTAG	GCCAGGACAATGCGACCGC	GAGACCCAGTACTTCGGG	Vβ29.1 Dβ1 Jβ2.5
12 S	TGTGCCACCTGGGATA	A	AAACTCTTT	Vγ8.1 Jγ2.1
12 LN	TGTGCCACCTGGGATA	A	AAACTCTTT	Vγ8.1 Jγ2.1
15	TGCGCCAGCAGT	TTACTAAAACCAC	CTAACTATGGCTACACC	Vβ10.1 Dβ 2 Jβ1.2
16	TTGATTTACTTC	CAGGGTAACAGT	CAACTAGACAAA	Vβ7.2 Dβ2 Jβ1.3
17	TGTGCCAGTCA	TAAAGGGAGGGT	CTGATCTCTACG	Vβ30.4 Dβ2 Jβ2.1
18	CAGTA	TTAGGCTAGCGGG	CCTACGACAGTACTTCGG	Vβ29.1 Dβ2 Jβ2.7
19	TGTGCCAGTAGT	TCGGC	AGCAATCAGCCCCAGCATT	Vβ7.2 Dβ1 Jβ1.5
20	TGTGCCACCTGGGATGGG	C	TATAAGAAACTCTTT	Vγ4.2 Jγ2.1
23	TGTGCCAGCAGC	CCGCTACAGGGA	TCCTACGAGCAGTACTTCG	Vβ7.9 Dβ2 Jβ2.7
25*	TGTGATCTTGCTGAAGGAAGTA		GACTAAGCTCATAGTAACT	Vγ4.1 JPγ1.1
25*	TGTGCCACCTGGGATAGG		ATTATTATAAGAAACTCTTT	Vγ8.1 Jγ2.1
26 S	TGTGCCAGCAGC	TGGGACAGGGGTAG	CTCCTACGAGCAGTACCAA	Vβ5.4 Dβ1 Jβ2.7
26 LN	TGTGCCAGCAGC	TGGGACAGGGGTAG	CTCCTACGAGCAGTACCAA	Vβ5.4 Dβ1 Jβ2.7
27	TGTGATCTTGCTGAAGGAAGTA		ATTATTATAAGAAACTCT	Vγ4.1 J γ2.1
28	CAGCTT	CGACAGGGC	CAGATACGCAGTATTTTGG	Vβ13.2 Dβ1 Jβ2.3
30	TTCTACATCTGCAGTGCTAGAG	CTACGGCGGTAGCCTAT	GAGACCCAGTACTCGGGC	Vβ30.5 Dβ2 Jβ2.5
31	AGTGCTGACTAGCGG	GGCTGGGGG	AGACCCAGTACTTCC	Vβ20.1 Dβ2 Jβ2-5
32	AGCACC	AGTATCGCTAGCTCA	ACAATGAGCAGTTCTTCGG	Vβ30.4 Dβ2 Jβ2.1
33 S	TGTGCCACCTGGGATA	AGGAC	TATTATAAGAAACTCTTT	V γ8.1 J γ2.1
33 LN	TGTGCCACCTGGGA	AGGAC	TATTATAAGAAACTCTTT	Vγ8.1 J γ2.1
35	TGTGCCACC	CC	TTATTATAAGAAACTCTTT	V γ4.1 J γ2.1
MOLT†	TTATTATAAGAAACTCTTT	AGAGTCGACTAGCGATCCAAAA	AATGAGCAGTTCTTCGGG	Vβ20.1 Dβ2 J β2.1
HUT†	TCTGCAGCGT	AGGCAGGAGGCGGTTGGC	GGCTACACCTTCGGTTC	Vβ30 Dβ2 J β1.2

S indicates DNA obtained from skin biopsy; LN, DNA obtained from lymph node biopsy.

Blank cells indicate no identifiable/truncated junctional region.

Biallelic rearrangement.

†

T-cell lines as positive controls.

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