Table 2. Effect of SCF and IFN-γ, IL-4,... | American Society of Hematology

Table 2.

Effect of SCF and IFN-γ, IL-4, or IL-5 on human mast cells after 8 weeks of culture

After 8-wk culture	SCF	SCF + IFN-γ	SCF + IL-4	SCF + IL-5
No. cells	13.2 ± 2.1 × 10⁶	0.4 ± 0.23 × 10⁶	2.0 ± 0.2 × 10⁶	7.2 ± 0.8 × 10⁶
No. mast cells	12.4 ± 3.1 × 10⁶	0	1.8 ± 0.5 × 10⁶	6.2 ± 0.7 × 10⁶
Total divisions	19	15	14	17
CD34 expression MFI	11.2 ± 2.3	11.5 ± 2.1	12.3 ± 2.5	11.3 ± 3.1
Kit expression MFI	665.8 ± 89.9	22.9 ± 5.3	10.0 ± 3.4	653 ± 92.8
FcγRI expression % positive	70.7	0.4	30.4	59.2
β-hex release % of total	27.8 ± 3.5	3.3 ± 1.3	12.6 ± 2.5	30.0 ± 2.1
Tryptase content pg/cell	11.3 ± 2.3	1.2 ± 0.2	5.8 ± 0.4	19.3 ± 1.6
Histamine content pg/cell	4.3 ± 0.6	0.6 ± 0.1	3.5 ± 0.5	3.1 ± 1.4
Morphology	Toluidine positive many granules	Toluidine negative few granules	Toluidine positive some granules	Toluidine positive many granules
Size (diameter)	10-12 μm	3-5 μm	5-7 μm	10-12 μm

After 8-wk culture	SCF	SCF + IFN-γ	SCF + IL-4	SCF + IL-5
No. cells	13.2 ± 2.1 × 10⁶	0.4 ± 0.23 × 10⁶	2.0 ± 0.2 × 10⁶	7.2 ± 0.8 × 10⁶
No. mast cells	12.4 ± 3.1 × 10⁶	0	1.8 ± 0.5 × 10⁶	6.2 ± 0.7 × 10⁶
Total divisions	19	15	14	17
CD34 expression MFI	11.2 ± 2.3	11.5 ± 2.1	12.3 ± 2.5	11.3 ± 3.1
Kit expression MFI	665.8 ± 89.9	22.9 ± 5.3	10.0 ± 3.4	653 ± 92.8
FcγRI expression % positive	70.7	0.4	30.4	59.2
β-hex release % of total	27.8 ± 3.5	3.3 ± 1.3	12.6 ± 2.5	30.0 ± 2.1
Tryptase content pg/cell	11.3 ± 2.3	1.2 ± 0.2	5.8 ± 0.4	19.3 ± 1.6
Histamine content pg/cell	4.3 ± 0.6	0.6 ± 0.1	3.5 ± 0.5	3.1 ± 1.4
Morphology	Toluidine positive many granules	Toluidine negative few granules	Toluidine positive some granules	Toluidine positive many granules
Size (diameter)	10-12 μm	3-5 μm	5-7 μm	10-12 μm

Data presented are mean ± SEM (n = 5).