Table 1. Primers used for amplification... | American Society of Hematology

Table 1.

Primers used for amplification and sequencing of the VKORC1 gene, fragment size, and polymerase chain reaction (PCR) conditions

Primer	Position	Sequence	Amplicon size	Annealing temperature
VKORC1 5′ UTR FW	(-)718 to (-)699	TGATCCGCTGGTCTCTAGGT	786 bp	59°C
VKORC1 5′ UTR REV	68-49	GAGAGCACTAAGCCCGTCAG
VKORC1 EX1 FW	(-)49 to (-)31	CTCCGTGGCTGGTTTTCTC	303 bp	57°C
VKORC1 EX1 REV	254-235	CCGATCCCAGACTCCAGAAT
VKORC1 EX2 FW	1120-1139	TGACATGGAATCCTGACGTG	361 bp	57°C
VKORC1 EX2 REV	1480-1463	GAGCTGACCAAGGGGGAT
VKORC1 EX3 FW	3348-3365	AGTGCCTGAAGCCCACAC	326 bp	57°C
VKORC1 EX3 REV	3673-3654	ACCCAGATATGCCCCCTTAG
VKORC1 3′ UTR FW	3534-3553	AGCCTGATGTGGCTCAGTTT	467 bp	58°C
VKORC1 3′ UTR REV	4000-3981	ATAACCACCCTTAAACGCAG

Primer	Position	Sequence	Amplicon size	Annealing temperature
VKORC1 5′ UTR FW	(-)718 to (-)699	TGATCCGCTGGTCTCTAGGT	786 bp	59°C
VKORC1 5′ UTR REV	68-49	GAGAGCACTAAGCCCGTCAG
VKORC1 EX1 FW	(-)49 to (-)31	CTCCGTGGCTGGTTTTCTC	303 bp	57°C
VKORC1 EX1 REV	254-235	CCGATCCCAGACTCCAGAAT
VKORC1 EX2 FW	1120-1139	TGACATGGAATCCTGACGTG	361 bp	57°C
VKORC1 EX2 REV	1480-1463	GAGCTGACCAAGGGGGAT
VKORC1 EX3 FW	3348-3365	AGTGCCTGAAGCCCACAC	326 bp	57°C
VKORC1 EX3 REV	3673-3654	ACCCAGATATGCCCCCTTAG
VKORC1 3′ UTR FW	3534-3553	AGCCTGATGTGGCTCAGTTT	467 bp	58°C
VKORC1 3′ UTR REV	4000-3981	ATAACCACCCTTAAACGCAG

Oligonucleotides are numbered according to the AY587020 sequence, the A of the ATG initiation codon is denoted as the nucleotide +1.