HUBEC coculture inhibits colony-forming cell differentiation compared with cytokines alone
. | No. of colony-forming cells, × 103 . | . | . | . | |||
|---|---|---|---|---|---|---|---|
| Condition . | CFU-GMs . | BFU-Es . | CFUs-Mix . | CFUs-Total . | |||
| BM | |||||||
| CD34+CD38-, day 0 | 6.3 ± 0.2 | 2.4 ± 1.3 | 1.1 ± 0.2 | 9.9 ± 2.5 | |||
| Day-7 HUBECs + GM36SF | 76.2 ± 27.0 | 8.6 ± 4.3 | 11.5 ± 2.5 | 96.4 ± 26.3 | |||
| Day-7 GM36SF | 411.8 ± 26.0 | 62.3 ± 28.6 | 54.8 ± 39.3 | 529.2 ± 92.6 | |||
. | No. of colony-forming cells, × 103 . | . | . | . | |||
|---|---|---|---|---|---|---|---|
| Condition . | CFU-GMs . | BFU-Es . | CFUs-Mix . | CFUs-Total . | |||
| BM | |||||||
| CD34+CD38-, day 0 | 6.3 ± 0.2 | 2.4 ± 1.3 | 1.1 ± 0.2 | 9.9 ± 2.5 | |||
| Day-7 HUBECs + GM36SF | 76.2 ± 27.0 | 8.6 ± 4.3 | 11.5 ± 2.5 | 96.4 ± 26.3 | |||
| Day-7 GM36SF | 411.8 ± 26.0 | 62.3 ± 28.6 | 54.8 ± 39.3 | 529.2 ± 92.6 | |||
The colony forming cell (CFC) activity of day-0 FACS-sorted human BM CD34+CD38- cells and their progeny following 7-day culture with HUBECs + GM36SF or GM36SF alone was measured (N = 6). Cells were collected and placed in methylcellulose colony-forming assay cultures as described in “Materials and methods.” At day 14, the number of CFCs were counted in triplicate in each condition. The values presented reflect the mean number of CFCs per culture condition ± standard deviations.