Table 3.

Analysis of competitive repopulation assays




No.

% Donor-derived bone marrow

T test, mean

F test, SD
Primary recipients     
    Wild-type   17   56.6 ± 9.6   .10   .01  
    Hmgb3-/Y  20   64.5 ± 18.9    
Secondary recipients     
    Wild-type   12   56.2 ± 6.3   .11   <.01  
    Hmgb3-/Y
 
19
 
63.5 ± 14.4
 

 

 



No.

% Donor-derived bone marrow

T test, mean

F test, SD
Primary recipients     
    Wild-type   17   56.6 ± 9.6   .10   .01  
    Hmgb3-/Y  20   64.5 ± 18.9    
Secondary recipients     
    Wild-type   12   56.2 ± 6.3   .11   <.01  
    Hmgb3-/Y
 
19
 
63.5 ± 14.4
 

 

 

Southern blot analyses were performed on genomic DNA isolated from bone marrow to determine the contribution to hematopoietic repopulation made by HSCs of either 129/SvJ wild-type or Hmgb3-/Y origin (see “Materials and methods”). For each set of primary and secondary recipients, the mean percent and standard deviation of donor (Hmgb3-/Y or 129/SvJ wild-type) derived bone marrow repopulation was determined as outlined in “Materials and methods.” Statistical analyses were performed using the Student t test (mean percent values) and F test (standard deviation).