Analysis of competitive repopulation assays
. | No. . | % Donor-derived bone marrow . | T test, mean . | F test, SD . |
---|---|---|---|---|
Primary recipients | ||||
Wild-type | 17 | 56.6 ± 9.6 | .10 | .01 |
Hmgb3-/Y | 20 | 64.5 ± 18.9 | ||
Secondary recipients | ||||
Wild-type | 12 | 56.2 ± 6.3 | .11 | <.01 |
Hmgb3-/Y | 19 | 63.5 ± 14.4 |
. | No. . | % Donor-derived bone marrow . | T test, mean . | F test, SD . |
---|---|---|---|---|
Primary recipients | ||||
Wild-type | 17 | 56.6 ± 9.6 | .10 | .01 |
Hmgb3-/Y | 20 | 64.5 ± 18.9 | ||
Secondary recipients | ||||
Wild-type | 12 | 56.2 ± 6.3 | .11 | <.01 |
Hmgb3-/Y | 19 | 63.5 ± 14.4 |
Southern blot analyses were performed on genomic DNA isolated from bone marrow to determine the contribution to hematopoietic repopulation made by HSCs of either 129/SvJ wild-type or Hmgb3-/Y origin (see “Materials and methods”). For each set of primary and secondary recipients, the mean percent and standard deviation of donor (Hmgb3-/Y or 129/SvJ wild-type) derived bone marrow repopulation was determined as outlined in “Materials and methods.” Statistical analyses were performed using the Student t test (mean percent values) and F test (standard deviation).