Table 2. Effect of FucTVI treatment on... | American Society of Hematology

Table 2.

Effect of FucTVI treatment on cord blood CD34⁺ cell homing to the bone marrow of NOD/SCID mice

	Cell source	No. of mice	Untreated	FucTVI-treated	P
Homed CD34⁺ cells per femur
Exp 1	CD34⁺	3/3	333 ± 39	335 ± 28	.71
Exp 2	CD34⁺	3/2	182 ± 12	151 ± 39
Exp 3	MNCs	4/4	534 ± 42	814 ± 50	.95
Exp 4	MNCs	3/3	483 ± 52	325 ± 48
Homed CD34⁺ CD38^lo/neg cells per femur
Exp 5	CD34⁺	3/3	288 ± 45	293 ± 24	.92
Exp 6	CD34⁺	3/2	137 ± 8	111 ± 23

	Cell source	No. of mice	Untreated	FucTVI-treated	P
Homed CD34⁺ cells per femur
Exp 1	CD34⁺	3/3	333 ± 39	335 ± 28	.71
Exp 2	CD34⁺	3/2	182 ± 12	151 ± 39
Exp 3	MNCs	4/4	534 ± 42	814 ± 50	.95
Exp 4	MNCs	3/3	483 ± 52	325 ± 48
Homed CD34⁺ CD38^lo/neg cells per femur
Exp 5	CD34⁺	3/3	288 ± 45	293 ± 24	.92
Exp 6	CD34⁺	3/2	137 ± 8	111 ± 23

Total MNCs (20 × 10⁶ cells/mouse) or CD34⁺ cells (5 × 10⁵ cells/mouse) purified from cord blood samples were incubated for 40 minutes at 37°C in the presence or absence of FucTVI and GDP-fucose. Cells were then washed and injected in sublethally irradiated (400 cGy) NOD/SCID mice. BM was harvested 12 to 16 hours after injection and analyzed for the content of human CD34⁺ cells or CD34⁺/CD38^lo/neg by FACS. Shown is the mean ± SEM number of total human CD34⁺ or CD34⁺/CD38^lo/neg cells recovered per femur in 6 independent experiments. The type of cells injected (total MNCs or purified CD34⁺ cells) and the number of mice per group in each experiment is indicated (total 36 mice). P values compare the mean number of untreated versus FucTVI-treated homed cells after pooling experiments 1 + 2, 3 + 4, and 5 + 6.

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