Table 1. Effect of FucTVI treatment on... | American Society of Hematology

Table 1.

Effect of FucTVI treatment on selectin binding and sLex expression by cord blood CD34⁺ cells

	P-selectin	E-selectin	sLex expression
Untreated cells
%	71 ± 2	71 ± 3	62 ± 2
MFI	61 ± 5	153 ± 21	132 ± 28
Treated cells
%	91 ± 1	98 ± 1	96 ± 1
MFI	84 ± 6	528 ± 65	293 ± 73
P
%	< .00001	< .00001	< .00001
MFI	< .00001	< .00001	.005
n	25	24	11

	P-selectin	E-selectin	sLex expression
Untreated cells
%	71 ± 2	71 ± 3	62 ± 2
MFI	61 ± 5	153 ± 21	132 ± 28
Treated cells
%	91 ± 1	98 ± 1	96 ± 1
MFI	84 ± 6	528 ± 65	293 ± 73
P
%	< .00001	< .00001	< .00001
MFI	< .00001	< .00001	.005
n	25	24	11

Cord blood CD34⁺ cells were purified and treated or not with rhFucTVI for 40 minutes at 37°C and then analyzed for P- and E-selectin binding, or sLex expression (HECA 452 clone). Data are expressed as mean percentage ± SEM of selectin binding or sLex-expressing CD34⁺ cells, and mean fluorescence intensity (MFI) ± SEM within the selectin binding or sLex-positive population. P values compare percentage or MFI means between FucTVI-treated and untreated cells.

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