CFC potential and phenotype of H1-derived CD34+ cells after in vitro culture in serum-free and serum-containing media
. | . | Feeder-free in vitro culture . | . | |
|---|---|---|---|---|
| Parameter . | Sorted CD34+ cells . | SFEM . | αMEM + 10% FBS . | |
| Expansion, fold change | ||||
| Total cells | NA | 4.6 ± 2.2 | 5.2 ± 1.5 | |
| Total CFCs | NA | 2.4 ± 1.3 | 4.3 ± 1.8 | |
| CFCs, absolute no./106 cells | ||||
| E-CFC | 640 ± 320 | 0 | 0 | |
| GEMM-CFC | 3400 ± 550 | 300 ± 220 | 0 | |
| GM-CFC | 4850 ± 1300 | 1600 ± 340 | 1500 ± 520 | |
| M-CFC | 9950 ± 1200 | 8800 ± 1500 | 15 500 ± 6170 | |
| Phenotype, % | ||||
| CD34 | 98.6 ± 1.5 | 69.2 ± 11.2 | 24.9 ± 10.5 | |
| CD45 | 12.6 ± 2.3 | 95.2 ± 7.3 | 97.8 ± 2.1 | |
| CD38 | 0.6 ± 0.4 | 1.8 ± 0.9 | 76.0 ± 9.4 | |
. | . | Feeder-free in vitro culture . | . | |
|---|---|---|---|---|
| Parameter . | Sorted CD34+ cells . | SFEM . | αMEM + 10% FBS . | |
| Expansion, fold change | ||||
| Total cells | NA | 4.6 ± 2.2 | 5.2 ± 1.5 | |
| Total CFCs | NA | 2.4 ± 1.3 | 4.3 ± 1.8 | |
| CFCs, absolute no./106 cells | ||||
| E-CFC | 640 ± 320 | 0 | 0 | |
| GEMM-CFC | 3400 ± 550 | 300 ± 220 | 0 | |
| GM-CFC | 4850 ± 1300 | 1600 ± 340 | 1500 ± 520 | |
| M-CFC | 9950 ± 1200 | 8800 ± 1500 | 15 500 ± 6170 | |
| Phenotype, % | ||||
| CD34 | 98.6 ± 1.5 | 69.2 ± 11.2 | 24.9 ± 10.5 | |
| CD45 | 12.6 ± 2.3 | 95.2 ± 7.3 | 97.8 ± 2.1 | |
| CD38 | 0.6 ± 0.4 | 1.8 ± 0.9 | 76.0 ± 9.4 | |
Data are presented as mean ± SD (n = 3). NA indicates not applicable.