Sorting and phenotype of H1-derived CD34+ cells
Parameter . | Mean ± SD . |
|---|---|
| No. hES (H1) cells plated, ×106 | 8.4 ± 1.4 |
| Presort cells | |
| Absolute no., ×106 | 82.7 ± 22.4 |
| % CD34 | 12.8 ± 4.7 |
| Sorted CD34+ cells | |
| Absolute no., ×106 | 8.7 ± 3.8 |
| Purity, % | 97.1 ± 0.8 |
| Recovery, %* | 84.5 ± 6.2 |
| Phenotype of CD34+ cells, % | |
| CD31 | 88.5 ± 3.5 |
| CD41a | 20.1 ± 10.3 |
| CD43 | 37.8 ± 10.1 |
| CD45 | 14.2 ± 8.6 |
| CD38 | 0.6 ± 0.8 |
| CD184 (CXCR4) | 35.2 ± 23.2 |
Parameter . | Mean ± SD . |
|---|---|
| No. hES (H1) cells plated, ×106 | 8.4 ± 1.4 |
| Presort cells | |
| Absolute no., ×106 | 82.7 ± 22.4 |
| % CD34 | 12.8 ± 4.7 |
| Sorted CD34+ cells | |
| Absolute no., ×106 | 8.7 ± 3.8 |
| Purity, % | 97.1 ± 0.8 |
| Recovery, %* | 84.5 ± 6.2 |
| Phenotype of CD34+ cells, % | |
| CD31 | 88.5 ± 3.5 |
| CD41a | 20.1 ± 10.3 |
| CD43 | 37.8 ± 10.1 |
| CD45 | 14.2 ± 8.6 |
| CD38 | 0.6 ± 0.8 |
| CD184 (CXCR4) | 35.2 ± 23.2 |
n = 9 independent experiments.
Percent recovery indicates (absolute number of CD34+ cells in CD34+ fraction/absolute number of CD34+ cells in presort fraction) × 100.