Quantitative PCR measurement of beta-globin and gamma-globin mRNA expression among pooled samples
. | EPO . | EST . | P . |
|---|---|---|---|
| Day 7 | |||
| Gamma-globin mRNA | 1 960 ± 1280 | 12 100 ± 8530 | .02 |
| Beta-globin mRNA | 41 200 ± 22900 | 10 400 ± 8050 | .02 |
| Total globin mRNA | 43 100 ± 24200 | 22 500 ± 16600 | .1 |
| Gamma/total, % | 4.9 ± 5.3 | 53.9 ± 11.5 | .00001 |
| Day 14 | |||
| Gamma-globin mRNA | 49.1 ± 36.8 | 1 590 ± 1120 | .01 |
| Beta-globin mRNA | 3 540 ± 3260 | 4 580 ± 4230 | .4 |
| Total globin mRNA | 3 590 ± 3300 | 6 170 ± 5350 | .2 |
| Gamma/total, % | 1.4 ± 1.1 | 25.8 ± 20.9 | .00001 |
. | EPO . | EST . | P . |
|---|---|---|---|
| Day 7 | |||
| Gamma-globin mRNA | 1 960 ± 1280 | 12 100 ± 8530 | .02 |
| Beta-globin mRNA | 41 200 ± 22900 | 10 400 ± 8050 | .02 |
| Total globin mRNA | 43 100 ± 24200 | 22 500 ± 16600 | .1 |
| Gamma/total, % | 4.9 ± 5.3 | 53.9 ± 11.5 | .00001 |
| Day 14 | |||
| Gamma-globin mRNA | 49.1 ± 36.8 | 1 590 ± 1120 | .01 |
| Beta-globin mRNA | 3 540 ± 3260 | 4 580 ± 4230 | .4 |
| Total globin mRNA | 3 590 ± 3300 | 6 170 ± 5350 | .2 |
| Gamma/total, % | 1.4 ± 1.1 | 25.8 ± 20.9 | .00001 |
Messenger RNA samples from erythroblasts cultured in EPO and EST isolated on days 7 and 14 were examined for beta- and gamma-globin mRNA expression using quantitative PCR. The mean gamma- and beta-globin and total (gamma + beta) mRNA copy numbers per cell with the percentage of gamma/total globin are shown. The mean ± standard deviation values from experiments performed on cells from 5 separate donors are shown. The P values comparing the EPO and EST data are on the right.