Primers used to construct the PS/FII chimeras GlaFII-ΔTSR-PS and GlaFII-PS
Primers . | Sequences . |
|---|---|
| EcoFII, s | 5′-CTCACTATAGGCTAGCCTCGAgaattcTTCGAAATGGCGCACGTCCGAGGCTTGCAG-3′ |
| GlaΔ1, a | 5′-GCATGGCAGAGGACTACACTGGTCTGGAATGGCTGTGTACTTGGCCCAGAACACATC-3′ |
| GlaΔ2, s | 5′-TCCACGGCTACGGATGTGTTCTGGGCCAAGTACACAGCCATTCCAGACCAGTGTAGT-3′ |
| Gla1, a | 5′-AGTGAATAACCCAGTTTGAAAAGAGCGAAGACAAACTGTGTACTTGGCCCAGAACACATC-3′ |
| Gla2, s | 5′-TCCACGGCTACGGATGTGTTCTGGGCCAAGTACACAGTTTGTCTTCGCTCTTTTCAAACT-3′ |
| Afl, a | 5′-AGTGTAACCTCCAGGGTAATTGACACAAAGCTGAGCACacatgtTCTCAGAGCATT-3′ |
Primers . | Sequences . |
|---|---|
| EcoFII, s | 5′-CTCACTATAGGCTAGCCTCGAgaattcTTCGAAATGGCGCACGTCCGAGGCTTGCAG-3′ |
| GlaΔ1, a | 5′-GCATGGCAGAGGACTACACTGGTCTGGAATGGCTGTGTACTTGGCCCAGAACACATC-3′ |
| GlaΔ2, s | 5′-TCCACGGCTACGGATGTGTTCTGGGCCAAGTACACAGCCATTCCAGACCAGTGTAGT-3′ |
| Gla1, a | 5′-AGTGAATAACCCAGTTTGAAAAGAGCGAAGACAAACTGTGTACTTGGCCCAGAACACATC-3′ |
| Gla2, s | 5′-TCCACGGCTACGGATGTGTTCTGGGCCAAGTACACAGTTTGTCTTCGCTCTTTTCAAACT-3′ |
| Afl, a | 5′-AGTGTAACCTCCAGGGTAATTGACACAAAGCTGAGCACacatgtTCTCAGAGCATT-3′ |
Sequences complementary to the prothrombin cDNA are shown in bold, and sequences complementary to the PS cDNA are shown in italics. For primer EcoFII, the sequences complementary to the pCI-neo vector, upstream of the initial codon of the PS cDNA, are in uppercase letters. Primer EcoFII contains an EcoRI restriction site (lowercase letters), and primer Afl contains an Af/III restriction site (lowercase letters) for cloning purposes. For primer pairs GlaΔ1/GlaΔ2 and Gla1/Gla2, the sequence underlined in each antisense primer is complementary to the sequence underlined in each sense primer. s indicates sense primer; a, antisense primer.