Clinical characteristics of induced human SCLtTA/BCR-ABL double-transgenic animals at time of death
Animal . | Clinical condition . | WBC/μL, neutrophils (%)* . | Hgb (g/dL) . | Spleen weight, mg . | Other organs involved, gross pathology/histology . | Time of analysis, d . |
|---|---|---|---|---|---|---|
| W98 | Moribund | 25 800 (33) | 10.5 | 410† | LN, skin, lung bleed, PB lymphoblasts | 29 |
| W107 | Moribund, tumor right cervival LN | 8 300 (32) | 12.5 | 240 | Axillary LN (923 mg), liver, lung bleed | 122 |
| W109 | Moribund | 3 900 (26) | ND | 310 | Liver, LN, lung bleed | 105 |
| W110 | Moribund | 29 200 (82) | 8.2 | 850 | Kidney, perinephric fluid, liver, skin, LN (all myeloid cells), brittle bones | 57 |
| W114 | Moribund | 18 500 (61) | 12 | 240† | LN, liver (myeloid cells), lung bleed, brittle bones | 46 |
| W118 | Leukocytosis | 15 500 (51) | 14 | 260 | Liver, pale BM | 43 |
| W119 | Leukocytosis, anemia | 70 000 (27) | 10.5 | 480 | LN, liver, pale BM, PB lymphoblasts (70%) | 34 |
| W184 | Moribund | > 30 000 (34) | ND | 130 | LN (mild), brittle bones | 67 |
| W185 | Neutrophilia, humanely killed | 9 200 (54) | ND | 300 | Pale BM | 42 |
| W194 | Found dead | 25 300 (ND) | ND | 440 | Kidney, liver | 66 |
| W202 | Neutrophilia, humanely killed | 9 500 (50) | ND | 270 | pale BM | 30 |
| W207 | Moribund, distended abdomen | 22 000 (53) | ND | 400 | Liver, kidney | 69 |
| W209 | Moribund | 19 600 (63) | ND | 130 | LN (mild) | 42 |
| W214 | Humanely killed | 6 800 (26) | ND | 120 | None | 7 |
Animal . | Clinical condition . | WBC/μL, neutrophils (%)* . | Hgb (g/dL) . | Spleen weight, mg . | Other organs involved, gross pathology/histology . | Time of analysis, d . |
|---|---|---|---|---|---|---|
| W98 | Moribund | 25 800 (33) | 10.5 | 410† | LN, skin, lung bleed, PB lymphoblasts | 29 |
| W107 | Moribund, tumor right cervival LN | 8 300 (32) | 12.5 | 240 | Axillary LN (923 mg), liver, lung bleed | 122 |
| W109 | Moribund | 3 900 (26) | ND | 310 | Liver, LN, lung bleed | 105 |
| W110 | Moribund | 29 200 (82) | 8.2 | 850 | Kidney, perinephric fluid, liver, skin, LN (all myeloid cells), brittle bones | 57 |
| W114 | Moribund | 18 500 (61) | 12 | 240† | LN, liver (myeloid cells), lung bleed, brittle bones | 46 |
| W118 | Leukocytosis | 15 500 (51) | 14 | 260 | Liver, pale BM | 43 |
| W119 | Leukocytosis, anemia | 70 000 (27) | 10.5 | 480 | LN, liver, pale BM, PB lymphoblasts (70%) | 34 |
| W184 | Moribund | > 30 000 (34) | ND | 130 | LN (mild), brittle bones | 67 |
| W185 | Neutrophilia, humanely killed | 9 200 (54) | ND | 300 | Pale BM | 42 |
| W194 | Found dead | 25 300 (ND) | ND | 440 | Kidney, liver | 66 |
| W202 | Neutrophilia, humanely killed | 9 500 (50) | ND | 270 | pale BM | 30 |
| W207 | Moribund, distended abdomen | 22 000 (53) | ND | 400 | Liver, kidney | 69 |
| W209 | Moribund | 19 600 (63) | ND | 130 | LN (mild) | 42 |
| W214 | Humanely killed | 6 800 (26) | ND | 120 | None | 7 |
Clinical condition refers to the condition of the animals at the time of analysis. Humanely killed indicates mice killed for analysis before the development of an obvious phenotype. All mice developed neutrophilia (defined as more than 20% neutrophils; mean neutrophil percentage of control animals was 10% ± 3% WBCs, and the neutrophil percentage in control animals did not exceed 20%) after induction of BCR-ABL. Platelet counts (normal range, 300-900/nL) were not significantly elevated. Normal spleen weight in single- transgenic animals was 80 to 90 mg (n = 3). Thirty-one percent of all animals demonstrated a biphasic phenotype, consisting of neutrophilia, subsequent development of lymphoid tumors, and acute lymphoblastic leukemia reminiscent of blast crisis. Hgb indicates hemoglobin (normal range, 12-16 g/dL); LN, lymphadenopathy; BM, bone marrow; PB, peripheral blood; ND, not determined at time of death.
Normal range of WBC counts was 4000 to 10,000/μL.
Myeloid cells.