Mg2+-loaded cell parameters in Hb AA and SS erythrocytes
[Mg2+]o, mM . | [Mg2+]T,i, mmol/L cell water . | pHo . | pHi . | r2 . | [Mg2+]i, mmol/L cell water . | MCV, fL . | [Na+]T,i, mmol/L cell . | [K+]T,i, mmol/L cell . |
|---|---|---|---|---|---|---|---|---|
| AA | ||||||||
| 0.2 ± 0.0 | 1.5 ± 0.2 | 7.21 ± 0.01 | 7.08 ± 0.01 | 1.77 | 0.4 ± 0.0 | 94 ± 1 | 5 ± 0.3 | 102 ± 4 |
| 1.1 ± 0.0 | 4.3 ± 0.2 | 7.20 ± 0.01 | 7.08 ± 0.01 | 1.70 | 1.9 ± 0.0 | 93 ± 1 | 5 ± 0.7 | 93 ± 6 |
| 2.0 ± 0.1 | 6.8 ± 0.3 | 7.19 ± 0.02 | 7.10 ± 0.02 | 1.50 | 3.0 ± 0.1 | 94 ± 1 | 5 ± 0.5 | 98 ± 8 |
| 3.2 ± 0.1 | 9.1 ± 0.5 | 7.18 ± 0.01 | 7.11 ± 0.01 | 1.41 | 4.6 ± 0.1 | 95 ± 1 | 5 ± 1.0 | 89 ± 2 |
| 4.0 ± 0.1 | 11.4 ± 0.1 | 7.17 ± 0.01 | 7.12 ± 0.01 | 1.28 | 5.2 ± 0.1 | 95 ± 2 | 5 ± 0.6 | 97 ± 9 |
| 8.1 ± 0.2 | 14.2 ± 1.0 | 7.16 ± 0.01 | 7.13 ± 0.02 | 1.17 | 9.5 ± 0.2 | 98 ± 2 | 5 ± 0.5 | 89 ± 5 |
| 9.8 ± 0.1 | 16.1 ± 1.0 | 7.16 ± 0.02 | 7.15 ± 0.01 | 1.07 | 10.5 ± 0.1 | 98 ± 3 | 5 ± 0.5 | 93 ± 6 |
| 12.2 ± 0.2 | 18.0 ± 1.0 | 7.15 ± 0.02 | 7.15 ± 0.01 | 0.98 | 11.9 ± 0.2 | 101 ± 4 | 5 ± 0.3 | 85 ± 6 |
| 14.6 ± 0.2 | 19.2 ± 1.0 | 7.15 ± 0.01 | 7.14 ± 0.01 | 1.01 | 14.8 ± 0.3 | 102 ± 3 | 5 ± 0.4 | 86 ± 8 |
| 16.6 ± 0.1 | 20.3 ± 1.0 | 7.17 ± 0.01 | 7.15 ± 0.01 | 0.98 | 17.7 ± 0.1 | 103 ± 4 | 5 ± 1.0 | 84 ± 13 |
| SS | ||||||||
| 0.2 ± 0.0 | 1.9 ± 0.1 | 7.01 ± 0.02 | 7.04 ± 0.01 | 0.89 | 0.2 ± 0.0 | 93 ± 8 | 12 ± 2 | 88 ± 7 |
| 0.8 ± 0.1 | 4.9 ± 0.3 | 7.02 ± 0.03 | 7.03 ± 0.02 | 0.94 | 0.7 ± 0.0 | 88 ± 7 | 10 ± 4 | 93 ± 4 |
| 1.5 ± 0.0 | 6.1 ± 0.1 | 7.02 ± 0.02 | 7.02 ± 0.02 | 0.98 | 1.5 ± 0.0 | 94 ± 8 | 10 ± 3 | 83 ± 6 |
| 2.6 ± 0.1 | 7.0 ± 0.3 | 6.98 ± 0.01 | 7.02 ± 0.02 | 0.87 | 2.3 ± 0.0 | 96 ± 6 | 10 ± 4 | 75 ± 9 |
| 3.3 ± 0.1 | 8.2 ± 0.6 | 6.99 ± 0.02 | 7.03 ± 0.02 | 0.85 | 2.8 ± 0.0 | 93 ± 7 | 11 ± 6 | 83 ± 8 |
| 6.7 ± 0.3 | 11.0 ± 1 | 6.99 ± 0.01 | 7.05 ± 0.03 | 0.76 | 5.1 ± 0.0 | 96 ± 8 | 11 ± 3 | 81 ± 6 |
| 8.1 ± 0.6 | 11.6 ± 0.2 | 6.95 ± 0.01 | 7.02 ± 0.01 | 0.72 | 5.9 ± 0.0 | 106 ± 8 | 15 ± 6 | 70 ± 7 |
| 10.6 ± 0.4 | 13.9 ± 1 | 6.98 ± 0.01 | 7.04 ± 0.01 | 0.75 | 8.0 ± 0.1 | 91 ± 7 | 13 ± 2 | 90 ± 11 |
| 13.1 ± 0.7 | 16.9 ± 1 | 6.94 ± 0.01 | 7.03 ± 0.01 | 0.67 | 8.8 ± 0.1 | 94 ± 7 | 14 ± 3 | 66 ± 9 |
| 17.9 ± 0.6 | 16.1 ± 1 | 6.97 ± 0.02 | 7.06 ± 0.02 | 0.68 | 12.1 ± 0.1 | 89 ± 6 | 14 ± 2 | 76 ± 5 |
[Mg2+]o, mM . | [Mg2+]T,i, mmol/L cell water . | pHo . | pHi . | r2 . | [Mg2+]i, mmol/L cell water . | MCV, fL . | [Na+]T,i, mmol/L cell . | [K+]T,i, mmol/L cell . |
|---|---|---|---|---|---|---|---|---|
| AA | ||||||||
| 0.2 ± 0.0 | 1.5 ± 0.2 | 7.21 ± 0.01 | 7.08 ± 0.01 | 1.77 | 0.4 ± 0.0 | 94 ± 1 | 5 ± 0.3 | 102 ± 4 |
| 1.1 ± 0.0 | 4.3 ± 0.2 | 7.20 ± 0.01 | 7.08 ± 0.01 | 1.70 | 1.9 ± 0.0 | 93 ± 1 | 5 ± 0.7 | 93 ± 6 |
| 2.0 ± 0.1 | 6.8 ± 0.3 | 7.19 ± 0.02 | 7.10 ± 0.02 | 1.50 | 3.0 ± 0.1 | 94 ± 1 | 5 ± 0.5 | 98 ± 8 |
| 3.2 ± 0.1 | 9.1 ± 0.5 | 7.18 ± 0.01 | 7.11 ± 0.01 | 1.41 | 4.6 ± 0.1 | 95 ± 1 | 5 ± 1.0 | 89 ± 2 |
| 4.0 ± 0.1 | 11.4 ± 0.1 | 7.17 ± 0.01 | 7.12 ± 0.01 | 1.28 | 5.2 ± 0.1 | 95 ± 2 | 5 ± 0.6 | 97 ± 9 |
| 8.1 ± 0.2 | 14.2 ± 1.0 | 7.16 ± 0.01 | 7.13 ± 0.02 | 1.17 | 9.5 ± 0.2 | 98 ± 2 | 5 ± 0.5 | 89 ± 5 |
| 9.8 ± 0.1 | 16.1 ± 1.0 | 7.16 ± 0.02 | 7.15 ± 0.01 | 1.07 | 10.5 ± 0.1 | 98 ± 3 | 5 ± 0.5 | 93 ± 6 |
| 12.2 ± 0.2 | 18.0 ± 1.0 | 7.15 ± 0.02 | 7.15 ± 0.01 | 0.98 | 11.9 ± 0.2 | 101 ± 4 | 5 ± 0.3 | 85 ± 6 |
| 14.6 ± 0.2 | 19.2 ± 1.0 | 7.15 ± 0.01 | 7.14 ± 0.01 | 1.01 | 14.8 ± 0.3 | 102 ± 3 | 5 ± 0.4 | 86 ± 8 |
| 16.6 ± 0.1 | 20.3 ± 1.0 | 7.17 ± 0.01 | 7.15 ± 0.01 | 0.98 | 17.7 ± 0.1 | 103 ± 4 | 5 ± 1.0 | 84 ± 13 |
| SS | ||||||||
| 0.2 ± 0.0 | 1.9 ± 0.1 | 7.01 ± 0.02 | 7.04 ± 0.01 | 0.89 | 0.2 ± 0.0 | 93 ± 8 | 12 ± 2 | 88 ± 7 |
| 0.8 ± 0.1 | 4.9 ± 0.3 | 7.02 ± 0.03 | 7.03 ± 0.02 | 0.94 | 0.7 ± 0.0 | 88 ± 7 | 10 ± 4 | 93 ± 4 |
| 1.5 ± 0.0 | 6.1 ± 0.1 | 7.02 ± 0.02 | 7.02 ± 0.02 | 0.98 | 1.5 ± 0.0 | 94 ± 8 | 10 ± 3 | 83 ± 6 |
| 2.6 ± 0.1 | 7.0 ± 0.3 | 6.98 ± 0.01 | 7.02 ± 0.02 | 0.87 | 2.3 ± 0.0 | 96 ± 6 | 10 ± 4 | 75 ± 9 |
| 3.3 ± 0.1 | 8.2 ± 0.6 | 6.99 ± 0.02 | 7.03 ± 0.02 | 0.85 | 2.8 ± 0.0 | 93 ± 7 | 11 ± 6 | 83 ± 8 |
| 6.7 ± 0.3 | 11.0 ± 1 | 6.99 ± 0.01 | 7.05 ± 0.03 | 0.76 | 5.1 ± 0.0 | 96 ± 8 | 11 ± 3 | 81 ± 6 |
| 8.1 ± 0.6 | 11.6 ± 0.2 | 6.95 ± 0.01 | 7.02 ± 0.01 | 0.72 | 5.9 ± 0.0 | 106 ± 8 | 15 ± 6 | 70 ± 7 |
| 10.6 ± 0.4 | 13.9 ± 1 | 6.98 ± 0.01 | 7.04 ± 0.01 | 0.75 | 8.0 ± 0.1 | 91 ± 7 | 13 ± 2 | 90 ± 11 |
| 13.1 ± 0.7 | 16.9 ± 1 | 6.94 ± 0.01 | 7.03 ± 0.01 | 0.67 | 8.8 ± 0.1 | 94 ± 7 | 14 ± 3 | 66 ± 9 |
| 17.9 ± 0.6 | 16.1 ± 1 | 6.97 ± 0.02 | 7.06 ± 0.02 | 0.68 | 12.1 ± 0.1 | 89 ± 6 | 14 ± 2 | 76 ± 5 |
Red cells were loaded as described in “Patients, materials, and methods.” The values are mean ± SE of 3 different experiments for normal and sickle erythrocytes. [Mg2+]o indicates extracellular Mg2+; [Mg2+]T,i, total intracellular Mg2+ concentration; pHo, extracellular pH; pHi, intracellular pH; [Mg2+]i, intracellular Mg2+; MCV, mean cellular volume; [Na+]T,i, total intracellular Na+ concentration; and [K+]T,i, total intracellular K+ concentration.