Absolute numbers of B-cell subpopulations in spleen and bone marrow
. | . | . | Bone marrow . | . | . | . | |||
|---|---|---|---|---|---|---|---|---|---|
. | n* . | Spleen† . | Pro-B cells† . | Pre-B cells† . | Immature B cells† . | Mature B cells† . | |||
| Mouse strain | NA | B220+ | B220+cμ− | B220+cμ+lgM− | lgM+lgD− | lgM+lgD+ | |||
| Btk+ | 14 (5) | 61.0 ± 9.3 | 2.6 ± 0.3 | 13.8 ± 1.1 | 3.9 ± 1.3 | 3.7 ± 0.7 | |||
| Btk− | 13 (5) | 11.9 ± 1.3 | 3.0 ± 0.3 | 11.9 ± 1.0 | 3.9 ± 0.3 | 2.0 ± 0.2 | |||
| K430R-Btk | 5 | 23.1 ± 4.5 | 2.8 ± 0.4 | 12.8 ± 2.0 | 3.8 ± 0.8 | 2.0 ± 0.7 | |||
| SLP-65− | 5 | 21.7 ± 5.0 | 3.0 ± 0.5 | 12.8 ± 2.3 | 3.1 ± 0.6 | 1.5 ± 0.4 | |||
| Btk−SLP-65− | 5 | 1.7 ± 0.3 | 3.7 ± 0.3 | 8.2 ± 1.6 | 1.1 ± 0.3 | 0.1 ± 0.0 | |||
| K430R-Btk/SLP-65− | 7 | 7.1 ± 1.6‡ | 2.8 ± 0.3 | 10.4 ± 1.6 | 2.6 ± 0.4 | 0.7 ± 0.2 | |||
. | . | . | Bone marrow . | . | . | . | |||
|---|---|---|---|---|---|---|---|---|---|
. | n* . | Spleen† . | Pro-B cells† . | Pre-B cells† . | Immature B cells† . | Mature B cells† . | |||
| Mouse strain | NA | B220+ | B220+cμ− | B220+cμ+lgM− | lgM+lgD− | lgM+lgD+ | |||
| Btk+ | 14 (5) | 61.0 ± 9.3 | 2.6 ± 0.3 | 13.8 ± 1.1 | 3.9 ± 1.3 | 3.7 ± 0.7 | |||
| Btk− | 13 (5) | 11.9 ± 1.3 | 3.0 ± 0.3 | 11.9 ± 1.0 | 3.9 ± 0.3 | 2.0 ± 0.2 | |||
| K430R-Btk | 5 | 23.1 ± 4.5 | 2.8 ± 0.4 | 12.8 ± 2.0 | 3.8 ± 0.8 | 2.0 ± 0.7 | |||
| SLP-65− | 5 | 21.7 ± 5.0 | 3.0 ± 0.5 | 12.8 ± 2.3 | 3.1 ± 0.6 | 1.5 ± 0.4 | |||
| Btk−SLP-65− | 5 | 1.7 ± 0.3 | 3.7 ± 0.3 | 8.2 ± 1.6 | 1.1 ± 0.3 | 0.1 ± 0.0 | |||
| K430R-Btk/SLP-65− | 7 | 7.1 ± 1.6‡ | 2.8 ± 0.3 | 10.4 ± 1.6 | 2.6 ± 0.4 | 0.7 ± 0.2 | |||
NA indicates not applicable.
Number of mice analyzed; the numbers in parentheses indicate that the B-cell population in spleen was analyzed in 5 mice per group.
Absolute number as average ± SEM (×106).
The absolute numbers of splenic B220+ cells in K430R-Btk/SLP-65− mice were significantly different from the values in SLP-65− mice (P = .008) and in Btk/SLP-65 double-deficient mice (P = .025).