Frequency of dystrophin and utrophin protein before transplantation, after G-CSF administration, and at necropsy in the muscle fibers of xmd HC transplant recipients as determined by immunofluorescence
. | % dystrophin-positive fibers . | . | . | . | . | |||
|---|---|---|---|---|---|---|---|---|
| Dystrophin background and recipient no. . | . | After HCT . | . | . | % utrophin-positive fibers before HCT . | |||
| . | Before HCT . | 3 mo . | After G-CSF . | Necropsy . | . | |||
| Low | ||||||||
| G019 | 0 | 0 | 0 | 0.07 | 90 | |||
| G123 | 0.23 | 0.08 | 0.41 | 0.03 | 75 | |||
| G141 | 0.05 | 0 | 0.04 | ND | 80 | |||
| G142 | 0.15 | 0.56 | 0.09 | ND | 85 | |||
| High | ||||||||
| G121 | 60 | ND | ND | 20 | 87 | |||
| G122 | 50 | ND | ND | 50 | 90 | |||
| G125 | 30 | ND | ND | 30 | 88 | |||
. | % dystrophin-positive fibers . | . | . | . | . | |||
|---|---|---|---|---|---|---|---|---|
| Dystrophin background and recipient no. . | . | After HCT . | . | . | % utrophin-positive fibers before HCT . | |||
| . | Before HCT . | 3 mo . | After G-CSF . | Necropsy . | . | |||
| Low | ||||||||
| G019 | 0 | 0 | 0 | 0.07 | 90 | |||
| G123 | 0.23 | 0.08 | 0.41 | 0.03 | 75 | |||
| G141 | 0.05 | 0 | 0.04 | ND | 80 | |||
| G142 | 0.15 | 0.56 | 0.09 | ND | 85 | |||
| High | ||||||||
| G121 | 60 | ND | ND | 20 | 87 | |||
| G122 | 50 | ND | ND | 50 | 90 | |||
| G125 | 30 | ND | ND | 30 | 88 | |||
HCT indicates hematopoietic cell transplantation; G-CSF, granulocyte colony-stimulating factor; and ND, not determined.
Dystrophin and utrophin expression were examined by immunofluorescence. Mouse anti–human dystrophin and rabbit anti–human utrophin antibodies were used.