Percentage expression of wild-type dystrophin in recipient dog muscles before and after experimental manipulations using real-time PCR
Recipient's ID no. . | Before HCT (± SE) . | After HCT (± SE) . | After G-CSF (± SE) . | Muscle fiber transplants (± SE) . | Adjacent 0 (± SE) . | Adjacent 1 (± SE) . | Adjacent 2 (± SE) . |
|---|---|---|---|---|---|---|---|
| G121 | 0.01 (0.01) | < 0.01 | NA | ND | ND | ND | ND |
| G122 | < 0.01 | 0.03 (0.04) | NA | ND | ND | ND | ND |
| G125 | 0.05 (0.08) | 0.21 (0.25) | NA | ND | ND | ND | ND |
| G019 | 0.03 (0.05) | 0.15 (0.04) | 0.39 (0.17) | ND | ND | ND | ND |
| G123 | 0.03 (0.03) | 0.03 (0.03) | 0.12 (0.04) | 0.19 (0.30) | ND | ND | ND |
| G141 | 0.69 (0.36) | 0.02 (0.03) | 0.03 (0.02) | 20.67 (5.1)* | 0.08 (0.05) | 0.16 (0.04) | 6.10 (2.8)* |
| G142 | 0.03 (0.02) | 0.05 (0.08) | 0.02 (0.01) | 0.02 (0.03) | 1.9 (0.68)* | 0.02 (0.06) | < 0.01 |
Recipient's ID no. . | Before HCT (± SE) . | After HCT (± SE) . | After G-CSF (± SE) . | Muscle fiber transplants (± SE) . | Adjacent 0 (± SE) . | Adjacent 1 (± SE) . | Adjacent 2 (± SE) . |
|---|---|---|---|---|---|---|---|
| G121 | 0.01 (0.01) | < 0.01 | NA | ND | ND | ND | ND |
| G122 | < 0.01 | 0.03 (0.04) | NA | ND | ND | ND | ND |
| G125 | 0.05 (0.08) | 0.21 (0.25) | NA | ND | ND | ND | ND |
| G019 | 0.03 (0.05) | 0.15 (0.04) | 0.39 (0.17) | ND | ND | ND | ND |
| G123 | 0.03 (0.03) | 0.03 (0.03) | 0.12 (0.04) | 0.19 (0.30) | ND | ND | ND |
| G141 | 0.69 (0.36) | 0.02 (0.03) | 0.03 (0.02) | 20.67 (5.1)* | 0.08 (0.05) | 0.16 (0.04) | 6.10 (2.8)* |
| G142 | 0.03 (0.02) | 0.05 (0.08) | 0.02 (0.01) | 0.02 (0.03) | 1.9 (0.68)* | 0.02 (0.06) | < 0.01 |
Muscle fiber transplants refer to muscle biopsy samples taken from original marrow donors and implanted into muscle of HC transplant recipients; adjacent 0, 1 and 2 refer to successive sections of recipient muscle taken distal from the site of the muscle fiber transplant.
For before HCT/after HCT, P = .6; before HCT/after G-CSF, P = .7; and after HCT/after G-CSF, P = .3. All P values reflect the average of all dogs.
HCT indicates hematopoietic cell transplantation; G-CSF, granulocyte colony stimulating factor; NA, not applicable; ND, not determined.
‡ The number of wild-type dystrophin mRNA molecules (those containing the exon 6/exon 7 splice junction) was first standardized relative to GAPDH mRNA molecules in the same samples and then expressed as a percentage relative to dystrophin expression in a wild-type dog (100%).
Statistically significant difference between sample and pre-HCT sample