Oligonucleotide primers and probes used for real-time quantitative PCR
Gene and oligonucleotide . | Sequence . | Position . |
|---|---|---|
| PAI-1 | ||
| Sense primer | 5′-ggc tga ctt cac gag tct ttc a-3′ | 11616-11637 |
| Antisense primer | 5′-ttc act ttc tgc agc gcc t-3′ | 11758-11780 |
| Probe | 5′ - (FAM) acc aag agc ctc tcc acg tcg cg (TAMRA) -3′ | 11782-11800 |
| Cyclophilin | ||
| Sense primer | 5′-gta cta tta gcc atg gtc aac ccc-3′ | 1648-1671 |
| Antisense primer | 5′-cag tca aag gag acg cgg cc-3′ | 1711-1727, 4178-4180 |
| Probe | 5′ - (FAM) cgt cga cgg cga gcc ctt g (TAMRA) -3′ | 1692-1710 |
| GAPDH | ||
| Sense primer | 5′-cca cat cgc tca gac acc at-3′ | 2171-2190 |
| Antisense primer | 5′-cca ggc gcc caa tac g-3′ | 2195-2217, 3850-3854 |
| Probe | 5′ - (FAM) aag gtg aag gtc gga gtc aac gga (TAMRA) -3′ | 3857-3872 |
Gene and oligonucleotide . | Sequence . | Position . |
|---|---|---|
| PAI-1 | ||
| Sense primer | 5′-ggc tga ctt cac gag tct ttc a-3′ | 11616-11637 |
| Antisense primer | 5′-ttc act ttc tgc agc gcc t-3′ | 11758-11780 |
| Probe | 5′ - (FAM) acc aag agc ctc tcc acg tcg cg (TAMRA) -3′ | 11782-11800 |
| Cyclophilin | ||
| Sense primer | 5′-gta cta tta gcc atg gtc aac ccc-3′ | 1648-1671 |
| Antisense primer | 5′-cag tca aag gag acg cgg cc-3′ | 1711-1727, 4178-4180 |
| Probe | 5′ - (FAM) cgt cga cgg cga gcc ctt g (TAMRA) -3′ | 1692-1710 |
| GAPDH | ||
| Sense primer | 5′-cca cat cgc tca gac acc at-3′ | 2171-2190 |
| Antisense primer | 5′-cca ggc gcc caa tac g-3′ | 2195-2217, 3850-3854 |
| Probe | 5′ - (FAM) aag gtg aag gtc gga gtc aac gga (TAMRA) -3′ | 3857-3872 |