B-cell development
. | . | . | . | CD19+, % . | . | |
|---|---|---|---|---|---|---|
| Mouse . | Construct . | CD45+, % . | GFP+in CD45+, % . | GFP+ . | GFP− . | |
| 1 | p53i | 72.3 | 7.3 | 48.7 | 44.2 | |
| 2 | p53i | 78.5 | 47.1 | 83.2 | 81.5 | |
| 3 | p53i | 17.9 | 76.8 | 78.0 | 80.9 | |
| 4 | p53i | 51.2 | 1.0 | 26.7 | 75.8 | |
| 5 | p53i | 66.8 | 12.3 | 80.1 | 76.3 | |
| 6 | p53i | nd | nd | nd | nd | |
| 7 | p53i | 73.9 | 3.7 | 75.9 | 62.2 | |
| 8 | Control | 68.5 | 8.2 | 27.7 | 44.8 | |
| 9 | Control | 73.9 | 12.0 | 69.8 | 73.9 | |
| 10 | Control | 10.0 | 73.1 | 63.6 | 67.6 | |
. | . | . | . | CD19+, % . | . | |
|---|---|---|---|---|---|---|
| Mouse . | Construct . | CD45+, % . | GFP+in CD45+, % . | GFP+ . | GFP− . | |
| 1 | p53i | 72.3 | 7.3 | 48.7 | 44.2 | |
| 2 | p53i | 78.5 | 47.1 | 83.2 | 81.5 | |
| 3 | p53i | 17.9 | 76.8 | 78.0 | 80.9 | |
| 4 | p53i | 51.2 | 1.0 | 26.7 | 75.8 | |
| 5 | p53i | 66.8 | 12.3 | 80.1 | 76.3 | |
| 6 | p53i | nd | nd | nd | nd | |
| 7 | p53i | 73.9 | 3.7 | 75.9 | 62.2 | |
| 8 | Control | 68.5 | 8.2 | 27.7 | 44.8 | |
| 9 | Control | 73.9 | 12.0 | 69.8 | 73.9 | |
| 10 | Control | 10.0 | 73.1 | 63.6 | 67.6 | |
Newborn mice were injected with CD34+ fetal liver cells transduced with p53i siRNA-GFP or control-GFP constructs. Mice were killed, and the BM was analyzed using FACS. Percentages of CD19+ cells in the GFP+ and GFP− gates are given. Percentages of CD45+ cells and the proportion of GFP+ cells in this gate are also indicated.