Telomere dynamics of FANCG knock-down wild-type and G5 Terc−/− MEFs
Genotype . | shRNA (%RNA)* . | Telomere length a.u.f. (kbp) . | Signal-free ends, % . | RT-like fusions per metaphase . |
|---|---|---|---|---|
| Primary MEFs | ||||
| Wild-type | GFP shRNA (100) | 1516.3 ± 831.1 (40.7 ± 22.3) | 0.5 | 0 |
| FANCG shRNA3 (14.6 ± 4.8) | 1524.3 ± 808.3 (40.9 ± 21.7) | 0.2 | 0 | |
| G5 Terc−/− | GFP shRNA (100) | 1030.3 ± 815.3 (27.6 ± 21.8) | 4.7 | 0.08 |
| FANCG shRNA3 (11.9 ± 3.4) | 1002.2 ± 794.9 (26.9 ± 21.3) | 5.5 | 0.2 | |
| Immortalized MEFs | ||||
| Wild-type | GFP shRNA (100) | 1656.7 ± 916.0 (44.4 ± 24.5) | 0.8 | 0 |
| FANCG shRNA3 (30.2 ± 4.2) | 1456.7 ± 847.5 (39.1 ± 22.7) | 0.8 | 0 | |
| G5 Terc−/− | GFP shRNA (100) | 814.3 ± 757.1 (21.8 ± 20.3) | 10.3 | 4.88 |
| FANCG shRNA3 (16.2 ± 1.1) | 889.2 ± 829.2 (23.8 ± 22.2) | 12.1 | 3.93 |
Genotype . | shRNA (%RNA)* . | Telomere length a.u.f. (kbp) . | Signal-free ends, % . | RT-like fusions per metaphase . |
|---|---|---|---|---|
| Primary MEFs | ||||
| Wild-type | GFP shRNA (100) | 1516.3 ± 831.1 (40.7 ± 22.3) | 0.5 | 0 |
| FANCG shRNA3 (14.6 ± 4.8) | 1524.3 ± 808.3 (40.9 ± 21.7) | 0.2 | 0 | |
| G5 Terc−/− | GFP shRNA (100) | 1030.3 ± 815.3 (27.6 ± 21.8) | 4.7 | 0.08 |
| FANCG shRNA3 (11.9 ± 3.4) | 1002.2 ± 794.9 (26.9 ± 21.3) | 5.5 | 0.2 | |
| Immortalized MEFs | ||||
| Wild-type | GFP shRNA (100) | 1656.7 ± 916.0 (44.4 ± 24.5) | 0.8 | 0 |
| FANCG shRNA3 (30.2 ± 4.2) | 1456.7 ± 847.5 (39.1 ± 22.7) | 0.8 | 0 | |
| G5 Terc−/− | GFP shRNA (100) | 814.3 ± 757.1 (21.8 ± 20.3) | 10.3 | 4.88 |
| FANCG shRNA3 (16.2 ± 1.1) | 889.2 ± 829.2 (23.8 ± 22.2) | 12.1 | 3.93 |
a.u.f. indicates arbitrary units of fluorescence.
Average and standard deviation of 2 Independent real-time RT-PCR assays; MEFs of the same genotype assayed in parallel are considered as 100% FANCG RNA.