CD55 and CD59 on PNH patient RBCs at intervals following transfusion
. | Start . | 1 h after transfusion . | 1-2 d after transfusion . | 1 wk after transfusion . | 2 wk after transfusion . |
|---|---|---|---|---|---|
| Patient 1 | |||||
| CD59, % | 62 ± 2.2 | ND | 92 ± 1.2 | 61 ± 2.2 | ND |
| CD55, % | 46 ± 0.4 | ND | 49 ± 2.4 | 82 ± 1.0 | ND |
| Hgb, g/dL | 5g/dL | 8.2 | 8.5 | 8.6 | ND |
| Reticulocytes/μL | 200 000 | ND | 222 000 | ND | ND |
| Group A1, % | 96 ± 0.6 | 55 ± 2.8 | 55 ± 1.4 | 68 ± 2.6 | ND |
| Patient 2 | |||||
| CD59, % | 39 ± 0.4 | 40 ± 1.0 | 79 ± 0.7 | 74 ± 0.2 | 35 ± 0.0 |
| CD55, % | 83 ± 1.5 | ND | 99 ± 0.0 | 99 ± 0.2 | 90 ± 0.0 |
| Hgb, g/dL | 6.3 | 10.0 | 10.2 | 9.8 | 10.6 |
| Reticulocytes/μL | 245 000 | ND | 176,000 | ND | ND |
| Group A1, % | 99 ± 0.4 | 54 ± 3.0 | 52 ± 1.4 | 52 ± 1.0 | 74 ± 4.3 |
| Patient 3 | |||||
| CD59, % | 15 ± 0.3 | 17 ± 1.2 | 83 ± 0.0 | 87 ± 0.7 | 83 ± 0.7 |
| CD55, % | 32 ± 0.7 | 26 ± 1.4 | 34 ± 2.8 | 31 ± 0.7 | 40 ± 0.8 |
| Hgb, g/dL | 8.9 | 12.2 | 12.5 | 12.3 | 12.0 |
| Reticulocytes/μL | 298 000 | ND | ND | ND | ND |
| Group A1, % | 90 ± 0.5 | 51 ± 2.5 | 57 ± 1.9 | 54 ± 2.2 | 58 ± 4.6 |
| Patient 4 | |||||
| CD59, % | 26 ± 3.8 | 25 ± 2.0 | ND | 67 ± 0.0 | 97 ± 0.5 |
| CD55, % | 73 ± 3.2 | 72 ± 1.5 | ND | 66 ± 2.3 | 67 ± 1.5 |
| Hgb, g/dL | 5.0 | 8.2 | ND | 8.8 | 9.0 |
| Reticulocytes/μL | 24 000 | ND | ND | ND | ND |
| Group A1, % | 99 ± 2.5 | 54 ± 2.0 | ND | 55 ± 5.4 | 59 ± 2.5 |
| Patient 5 | |||||
| CD59, % | 56 ± 0.2 | 52 ± 0.3 | 97 ± 1.0 | 83 ± 1.7 | 86 ± 0.0 |
| CD55, % | 32 ± 1.4 | 38 ± 0.5 | 41 ± 2.0 | 45 ± 0.3 | 42 ± 0.6 |
| Hgb, g/dL | 6.2 | 9.0 | 9.2 | 9.5 | ND |
| Reticulocytes/μL | 54 000 | ND | 24 000 | ND | ND |
| Group A1, % | 99 ± 0.0 | 62 ± 2.4 | 66 ± 2.5 | 63 ± 2.4 | 69 ± 1.2 |
| Patient 6* | |||||
| CD59, % | 26 ± 1.5 | 11 ± 0.6 | ND | 26 ± 1.4 | 53 ± 0.8 |
| CD55, % | 44 ± 5.0 | 42 ± 0.7 | ND | 42 ± 0.6 | 61 ± 0.6 |
| Hgb, g/dL | 6.5 | 9.4 | 10.0 | 10.1 | 9.8 |
| Reticulocytes/μL | 233 000 | ND | 150 000 | ND | ND |
| Group A1, % | 97 ± 5.8 | 41 ± 3.4 | ND | 60 ± 2.2 | 61 ± 5.2 |
. | Start . | 1 h after transfusion . | 1-2 d after transfusion . | 1 wk after transfusion . | 2 wk after transfusion . |
|---|---|---|---|---|---|
| Patient 1 | |||||
| CD59, % | 62 ± 2.2 | ND | 92 ± 1.2 | 61 ± 2.2 | ND |
| CD55, % | 46 ± 0.4 | ND | 49 ± 2.4 | 82 ± 1.0 | ND |
| Hgb, g/dL | 5g/dL | 8.2 | 8.5 | 8.6 | ND |
| Reticulocytes/μL | 200 000 | ND | 222 000 | ND | ND |
| Group A1, % | 96 ± 0.6 | 55 ± 2.8 | 55 ± 1.4 | 68 ± 2.6 | ND |
| Patient 2 | |||||
| CD59, % | 39 ± 0.4 | 40 ± 1.0 | 79 ± 0.7 | 74 ± 0.2 | 35 ± 0.0 |
| CD55, % | 83 ± 1.5 | ND | 99 ± 0.0 | 99 ± 0.2 | 90 ± 0.0 |
| Hgb, g/dL | 6.3 | 10.0 | 10.2 | 9.8 | 10.6 |
| Reticulocytes/μL | 245 000 | ND | 176,000 | ND | ND |
| Group A1, % | 99 ± 0.4 | 54 ± 3.0 | 52 ± 1.4 | 52 ± 1.0 | 74 ± 4.3 |
| Patient 3 | |||||
| CD59, % | 15 ± 0.3 | 17 ± 1.2 | 83 ± 0.0 | 87 ± 0.7 | 83 ± 0.7 |
| CD55, % | 32 ± 0.7 | 26 ± 1.4 | 34 ± 2.8 | 31 ± 0.7 | 40 ± 0.8 |
| Hgb, g/dL | 8.9 | 12.2 | 12.5 | 12.3 | 12.0 |
| Reticulocytes/μL | 298 000 | ND | ND | ND | ND |
| Group A1, % | 90 ± 0.5 | 51 ± 2.5 | 57 ± 1.9 | 54 ± 2.2 | 58 ± 4.6 |
| Patient 4 | |||||
| CD59, % | 26 ± 3.8 | 25 ± 2.0 | ND | 67 ± 0.0 | 97 ± 0.5 |
| CD55, % | 73 ± 3.2 | 72 ± 1.5 | ND | 66 ± 2.3 | 67 ± 1.5 |
| Hgb, g/dL | 5.0 | 8.2 | ND | 8.8 | 9.0 |
| Reticulocytes/μL | 24 000 | ND | ND | ND | ND |
| Group A1, % | 99 ± 2.5 | 54 ± 2.0 | ND | 55 ± 5.4 | 59 ± 2.5 |
| Patient 5 | |||||
| CD59, % | 56 ± 0.2 | 52 ± 0.3 | 97 ± 1.0 | 83 ± 1.7 | 86 ± 0.0 |
| CD55, % | 32 ± 1.4 | 38 ± 0.5 | 41 ± 2.0 | 45 ± 0.3 | 42 ± 0.6 |
| Hgb, g/dL | 6.2 | 9.0 | 9.2 | 9.5 | ND |
| Reticulocytes/μL | 54 000 | ND | 24 000 | ND | ND |
| Group A1, % | 99 ± 0.0 | 62 ± 2.4 | 66 ± 2.5 | 63 ± 2.4 | 69 ± 1.2 |
| Patient 6* | |||||
| CD59, % | 26 ± 1.5 | 11 ± 0.6 | ND | 26 ± 1.4 | 53 ± 0.8 |
| CD55, % | 44 ± 5.0 | 42 ± 0.7 | ND | 42 ± 0.6 | 61 ± 0.6 |
| Hgb, g/dL | 6.5 | 9.4 | 10.0 | 10.1 | 9.8 |
| Reticulocytes/μL | 233 000 | ND | 150 000 | ND | ND |
| Group A1, % | 97 ± 5.8 | 41 ± 3.4 | ND | 60 ± 2.2 | 61 ± 5.2 |
Numbers for CD55, CD59, and A1 represent the mean and SD for at least 3 samples run at each time period.
ND indicates not determined; Hgb, hemoglobin.
Patient was also receiving corticosteroids for active hemolysis