Table 2. Clonal analysis of BL and NK... | American Society of Hematology

Table 2.

Clonal analysis of BL and NK cell differentiation potential of CD45RA^hiCD7⁺ and CD45RA^hiLin⁻CD10⁺ HPCs

	CD34⁺HPC populations
	CD45RA^hiCD7⁺	CD45RA^hiLin⁻CD10⁺
No. cells seeded	418	148
Total no. of clones	58	11
NK clones, %	43	9
B clones, %	7	63
Mixed B/NK clones, %	24	0
CD19⁻CD56⁻ clones, %	26	28

	CD34⁺HPC populations
	CD45RA^hiCD7⁺	CD45RA^hiLin⁻CD10⁺
No. cells seeded	418	148
Total no. of clones	58	11
NK clones, %	43	9
B clones, %	7	63
Mixed B/NK clones, %	24	0
CD19⁻CD56⁻ clones, %	26	28

Cells were seeded individually in MS5 cell-precoated wells and cultured for 3 weeks with SCF, TPO, IL2, and IL7. Clones were then harvested, labeled with CD56-PE and CD19-FITC mAbs, and analyzed by FACS. Data are pooled from 2 independent experiments.

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