Target ligand expression on EC-RF24 cells and HUVECs by flow cytometry
Antibody (target) . | % Positive EC-RF24 cells (MFI) . | % Positive HUVECs (MFI) . |
|---|---|---|
| P3 (negative control) | 1 (6) | 1 (8) |
| 7E3 (αvβ3 + αIIbβ3) | 90 (117) | 53 (101) |
| LM609 (αvβ3) | 89 (112) | 48 (81) |
| 10E5 (αIIbβ3) | 2 (26) | 2 (12) |
| 1951Z-20 (β1 integrin) | 99 (119) | 63 (96) |
| A3D8 (CD44) | 81 (233) | 67 (316) |
| MP30-1 (CD4-7) | 99 (115) | 39 (51) |
| Rabbit antilaminin | 52 (56) | 7 (9) |
| BS46 (LW) | 1 (6) | 1 (21) |
Antibody (target) . | % Positive EC-RF24 cells (MFI) . | % Positive HUVECs (MFI) . |
|---|---|---|
| P3 (negative control) | 1 (6) | 1 (8) |
| 7E3 (αvβ3 + αIIbβ3) | 90 (117) | 53 (101) |
| LM609 (αvβ3) | 89 (112) | 48 (81) |
| 10E5 (αIIbβ3) | 2 (26) | 2 (12) |
| 1951Z-20 (β1 integrin) | 99 (119) | 63 (96) |
| A3D8 (CD44) | 81 (233) | 67 (316) |
| MP30-1 (CD4-7) | 99 (115) | 39 (51) |
| Rabbit antilaminin | 52 (56) | 7 (9) |
| BS46 (LW) | 1 (6) | 1 (21) |
Indirect immunofluorescence assays were performed on EC-RF24 cells and HUVECs. 7E3 (anti-αVβ3 and -αIIβ3), LM609 (anti-αvβ3), 10E5 (anti-αIIbβ3), 1951Z-20 (anti-β1), A3D8 (anti-CD44), MP30-1 (anti-CD47), and anti-LW mouse antihuman mAbs were used at 5 μg/mL. P3 (as a negative control) was used as ascitic fluid diluted 1:500. Rabbit antihuman laminin mAb was diluted 1:500. Percentage and mean fluorescence intensity (MFI) of positive cells are shown.