Primer sequences, annealing temperatures, and cycle numbers
Gene . | Sense primer . | Antisense primer . | Annealing temp, °C . | Cycles . | Product size, bp . |
|---|---|---|---|---|---|
| RANKL | 5′-gccagtgggagatgttag-3′ | 5′-ttagctgcaagttttccc-3′ | 63 | 30 | 486 |
| OPG | 5′-gctaacctcaccttcgag-3′ | 5′-tgattggacctggttacc-3′ | 54 | 30 | 324 |
| TNF-α | 5′-tcagcctcttctccttcctg-3′ | 5′-gatgcggctgatggtgtg-3′ | 54 | 25 | 384 |
| MCSF | 5′-cagatggagacctcgtgccaa-3′ | 5′-gcaggccttgtcatgctcttc-3′ | 54 | 30 | 241 |
| FAS | 5′-ccaagtgactgacatcaactc-3′ | 5′-ctctttgcacttggtgttgctgg-3′ | 66 | 30 | 427 |
| FAS-L | 5′-gaaggagctggcagaactccgag-3′ | 5′-gaccagagagagctcagatacgttgac-3′ | 66 | 30 | 478 |
| TRAIL | 5′-caatgacgaagagagtatga-3′ | 5′-cccccttgatagatggaata-3′ | 54 | 30 | 536 |
| DcR1 | 5′-gccggaagtgtagcaggtg-3′ | 5′-gtagagtggatggtcagtg-3′ | 58 | 25 | 546 |
| DcR2 | 5′-cccccggcaggacgaagtt-3′ | 5′-ctcctccgctgctggggtttt-3′ | 58 | 25 | 418 |
| DR4 | 5′-ccgcggccacacccagaaagt-3′ | 5′-gtacatgggaggcaagcaaacaaa-3′ | 58 | 25 | 415 |
| DR5 | 5′-gcgcccacaaaatacaccgacgat-3′ | 5′-gcagcgcaagcagaaaaggag-3′ | 58 | 25 | 437 |
| Bcl-2 | 5′-gatgtccagccagctgcacctg-3′ | 5′-cacaaaggcatcccagcctcc-3′ | 63 | 25 | 255 |
| Bax | 5′-ggacccggtgcctcagga-3′ | 5′-caaagatggtcacggtctgc-3′ | 63 | 25 | 495 |
| Cathepsin K | 5′-tccatccataaccttgaggctt-3′ | 5′-ccacagccatcattctcagacaca-3′ | 65 | 20 | 360 |
| Integrin β 3 | 5′-gtgacctgaaggagaatctgc-3′ | 5′-ttcttcgaatcatctggcc-3′ | 65 | 35 | 183 |
| CTR | 5′-gtattgtcctatcagttctgcc-3′ | 5′-agagataataccaccgcaagcg-3′ | 65 | 35 | 577 |
| MMP-9 | 5′-ccatttcgacgatgacgagttg-3′ | 5′-cttgtcgctgtcaaagttcgag-3′ | 65 | 25 | 544 |
| c-fos | 5′-aaggagaatccgaagggaaaggaataagatggct-3′ | 5′-agacgaaggaagacgtgtaagcagtgcagct-3′ | 65 | 25 | 612 |
| GAPDH | 5′-ggagtcaacggatttggt-3′ | 5′-gtgatgggatttccattgat-3′ | 63 | - | 209 |
Gene . | Sense primer . | Antisense primer . | Annealing temp, °C . | Cycles . | Product size, bp . |
|---|---|---|---|---|---|
| RANKL | 5′-gccagtgggagatgttag-3′ | 5′-ttagctgcaagttttccc-3′ | 63 | 30 | 486 |
| OPG | 5′-gctaacctcaccttcgag-3′ | 5′-tgattggacctggttacc-3′ | 54 | 30 | 324 |
| TNF-α | 5′-tcagcctcttctccttcctg-3′ | 5′-gatgcggctgatggtgtg-3′ | 54 | 25 | 384 |
| MCSF | 5′-cagatggagacctcgtgccaa-3′ | 5′-gcaggccttgtcatgctcttc-3′ | 54 | 30 | 241 |
| FAS | 5′-ccaagtgactgacatcaactc-3′ | 5′-ctctttgcacttggtgttgctgg-3′ | 66 | 30 | 427 |
| FAS-L | 5′-gaaggagctggcagaactccgag-3′ | 5′-gaccagagagagctcagatacgttgac-3′ | 66 | 30 | 478 |
| TRAIL | 5′-caatgacgaagagagtatga-3′ | 5′-cccccttgatagatggaata-3′ | 54 | 30 | 536 |
| DcR1 | 5′-gccggaagtgtagcaggtg-3′ | 5′-gtagagtggatggtcagtg-3′ | 58 | 25 | 546 |
| DcR2 | 5′-cccccggcaggacgaagtt-3′ | 5′-ctcctccgctgctggggtttt-3′ | 58 | 25 | 418 |
| DR4 | 5′-ccgcggccacacccagaaagt-3′ | 5′-gtacatgggaggcaagcaaacaaa-3′ | 58 | 25 | 415 |
| DR5 | 5′-gcgcccacaaaatacaccgacgat-3′ | 5′-gcagcgcaagcagaaaaggag-3′ | 58 | 25 | 437 |
| Bcl-2 | 5′-gatgtccagccagctgcacctg-3′ | 5′-cacaaaggcatcccagcctcc-3′ | 63 | 25 | 255 |
| Bax | 5′-ggacccggtgcctcagga-3′ | 5′-caaagatggtcacggtctgc-3′ | 63 | 25 | 495 |
| Cathepsin K | 5′-tccatccataaccttgaggctt-3′ | 5′-ccacagccatcattctcagacaca-3′ | 65 | 20 | 360 |
| Integrin β 3 | 5′-gtgacctgaaggagaatctgc-3′ | 5′-ttcttcgaatcatctggcc-3′ | 65 | 35 | 183 |
| CTR | 5′-gtattgtcctatcagttctgcc-3′ | 5′-agagataataccaccgcaagcg-3′ | 65 | 35 | 577 |
| MMP-9 | 5′-ccatttcgacgatgacgagttg-3′ | 5′-cttgtcgctgtcaaagttcgag-3′ | 65 | 25 | 544 |
| c-fos | 5′-aaggagaatccgaagggaaaggaataagatggct-3′ | 5′-agacgaaggaagacgtgtaagcagtgcagct-3′ | 65 | 25 | 612 |
| GAPDH | 5′-ggagtcaacggatttggt-3′ | 5′-gtgatgggatttccattgat-3′ | 63 | - | 209 |
FAS indicates fatty acid synthase; FASL, Fas ligand; GAPDH, glyceraldehyde-3-phosphate dehydrogenase.