Table 4.

Expression levels of genes important for MK development

Description
GenBank accession no.
Normal MK signal
ET MK signal
SLR
Quantitative RT-PCR SLR
Selected proteins      
    Glycoprotein Ib (platelet), alpha polypeptide NM_000173.1  3028.7, P   3630.25, P   0.28, NC   1.5  
    Glycoprotein VI (platelet)  AB043821.1   314.9, P   381.1, P   0.22, NC   NA  
    Glycoprotein IX (platelet) NM_000174.1  983.3, P   619.7, P   -0.54, NC   NA  
    Integrin, alpha 2b (platelet glycoprotein IIb of IIb/IIIa complex, antigen CD41B) NM_000419.2  3829.7, P   4136.4, P   0.33, NC   1.1  
    Integrin, beta 3 (platelet glycoprotein IIIa, antigen CD61)  AI189839   469.9, P   288.8, P   -0.38, NC   1.5  
    CD9 antigen (p24) NM_001769.1  2652.9, P   2360, P   -0.17, NC   NA  
Inhibitor      
    Transforming growth factor, beta 1 (Camurati-Engelmann disease)   BC000125.1   2953.9, P   3440.9, P   0.2, NC   1.1  
Growth factors      
    Interleukin 1, beta  M15330   208.4, P   154.6, P   -0.3, NC   NA  
    Interleukin 1, beta NM_000576.1  329.4, P   285, P   -0.27, NC   NA  
    Interleukin 6 (interferon, beta 2) NM_000600.1  —, A   16.9, P   -0.11, NC   NA  
    Interleukin 11 NM_000641.1  —, A   —, A   —, NC   NA  
    Platelet-derived growth factor alpha polypeptide NM_002607.1  223.9, P   267.5, P   0.4, I   1.5  
    Platelet-derived growth factor beta polypeptide  AK022920.1   21.7, P   23.7, P   -0.26, NC   NA  
    Platelet-derived growth factor C NM_016205.1  651.6, P   708.8, P   0.21, NC   1.4  
    Vascular endothelial growth factor  AF022375.1   568.6, P   467.2, P   -0.32, NC   NA  
Growth factors receptors      
    Interleukin 1 receptor NM_000877.1  —, A   —, A   —, NC   NA  
    Interleukin 3 receptor NM_002183.1  —, A   —, A   —, NC   NA  
    Interleukin 6 receptor NM_000565.1  67.1, P   43.2, P   -0.69, NC   0.1  
    Interleukin 11 receptor NM_004512.1  —, A   —, A   —, NC   NA  
    Myeloproliferative leukemia virus oncogene NM_005373.1  474.8, P   554.7, P   -0.01, NC   NA  
    V-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene homolog NM_000222.1  223.7, P   165.1, P   -0.9, D   NA  
    Chemokine (C-X-C motif) receptor 4  AJ224869   1505.5, P   957.7, P   -0.48, NC   1.0  
    Thromboxane A2 receptor  D38081   653.6, P   690.8, P   0.38, NC   1.5  
Cell cycle regulators      
    Cyclin B1  BE407516   128.6, P   159.5, P   0.15, NC   NA  
    Cyclin D3 NM_001760.1  3247.9, P   3241.9, P   0, NC   0.7  
    Cyclin E1  AI671049   31.1, P   —, A   -0.52, NC   NA  
    Cyclin E2 NM_004702.1  44.7, P   34, P   -0.24, NC   NA  
    Cell division cycle 2, G1 to S and G2 to M  AL524035   287, P   136.8, P   -0.65, D   NA  
    Cyclin-dependent kinase 2  M68520.1   240.9, P   172.4, P   -0.12, NC   NA  
    Cyclin-dependent kinase inhibitor 1B (p27, Kip1)  BC001971.1   309, P   232.5, P   -0.44, NC   NA  
    RAS p21 protein activator (GTPase activating protein) 1  M23612.1   339.9, P   271.2, P   -0.45, D   NA  
    Cyclin-dependent kinase inhibitor 2A (melanoma, p16, inhibits CDK4 NM_000077.1  —, A   —, A   —, NC   NA  
    RAS guanyl releasing protein 2 (calcium and DAG-regulated) NM_005825.1  637.9, P   868.2, P   0.36, NC   1.6  
Apoptosis regulators      
    BCL2-antagonist of cell death  U66879   37.6, P   23, A   -0.02, NC   NA  
    BCL2-antagonist/killer 1 NM_001188.1  66.2, P   82.1, P   -0.13, NC   NA  
    BCL2-like 2  D87461.1   112, P   174.2, P   0.42, NC   NA  
    B-cell CLL/lymphoma 2 NM_000633.1  16.6, P   21.1, P   0.3, NC   NA  
    Caspase-3, apoptosis-related cysteine protease NM_004346.1  115.3, P   155.4, P   0.53, I   1.7  
    Caspase-9, apoptosis-related cysteine protease  U60521.1   171.8, P   196.5, P   0.07, NC   0.9  
    Myeloid cell leukemia sequence 1 (BCL2-related) NM_021960.1  874.6, P   1078.7, P   0.3, NC   1.5  
Transcriptional regulators      
    Friend leukemia virus integration 1 NM_002017.2  538.1, P   322, P   -0.52, D   NA  
    GATA binding protein 1 (globin transcription factor 1)  M30601.1   606, P   677.6, P   0.18, NC   NA  
    GATA binding protein 2 NM_002050.1  67.7, P   83.2, P   -0.09, NC   NA  
    Jumonji homolog (mouse)  BG029530   1139.2, P   911.1, P   0.19, NC   NA  
    Jun D proto-oncogene NM_005354.2  2139.5, P   2371.8, P   0.24, NC   1.1  
    V-jun sarcoma virus 17 oncogene homolog (avian)  BE327172   8.45, P   27.6, P   1.26, NC   NA  
    Kruppel-like factor 1 (erythroid)  U65404.1   96, P   83, P   0.05, NC   NA  
    V-maf musculoaponeurotic fibrosarcoma oncogene homolog G (avian) NM_002359.1  299.5, P   286.9, P   0.39, NC   NA  
    V-myb myeloblastosis viral oncogene homolog (avian) NM_005375.1  85.2, P   96.35, P   -0.16, NC   NA  
    V-myc myelocytomatosis viral oncogene homolog (avian) NM_002467.1  317.6, P   353.6, P   0.42, NC   NA  
    V-fos FBJ murine osteosarcoma viral oncogene homolog  BC004490.1   —, A   —, A   —, NC   0.0  
    Nuclear factor (erythroid-derived 2), 45 kDa  L13974.1   1386.8, P   1419.3, P   0.03, NC   1.1  
    T-cell acute lymphocytic leukemia 1 NM_003189.1  1873.2, P   1763.8, P   0.03, NC   1.1  
    Ecotropic viral integration site 1  S82592.1   —, A   —, A   —, NC   NA  
    Core-binding factor, runt domain, alpha subunit 2; translocated to 3 NM_005187.2  234.7, P   377.1, P   0.57, I   1.6  
    Core-binding factor, runt domain, alpha subunit 2; translocated to 2 NM_005093.1  69.1, P   80.3, P   0.33, NC   NA  
Chemokines      
    GRO1 oncogene (melanoma growth stimulating activity, alpha) NM_001511.1  20.65, P   157.8, P   2.33, I   3.2  
    Stromal cell-derived factor 1  U19495.1   30.8, P   62.9, P   0.5, I   2.6  
    Interleukin 8 NM_000584.1  435.8, P   1601.3, P   1.87, I   3.9  
    Proplatelet basic protein  R64130   4476.1, P   4161.7, P   0.06, NC   0.7  
    Small inducible cytokine A5 (Rantes)  M21121   302.1, P   524.3, P   0.44, NC   NA  
    Platelet factor 4 NM_002619.1  6642.2, P   7639.8, P   0.12, NC   0.7  
Signal transducers      
    Signal transducer and activator of transcription  M97935   148.8, P   132, P   0.05, NC   NA  
    Signal transducer and activator of transcription 3  BC000627.1   503.3, P   375.2, P   -0.46, NC   NA  
    Signal transducer and activator of transcription 5A NM_003152.1  229.9, P   248.5, P   0.11, NC   NA  
    Mitogen-activated protein kinase 14 NM_001315.1  681, P   535.2, P   -0.57, D   0.9  
    Janus kinase 2 (a protein tyrosine kinase) NM_004972.2  45.5, P   34.6, P   -0.08, NC   NA  
    Phosphoinositide-3-kinase, class 2, beta polypeptide
 		NM_002646.1
 		 —, A
 		 —, A
 		 —, NC
 		 NA
 
Description
GenBank accession no.
Normal MK signal
ET MK signal
SLR
Quantitative RT-PCR SLR
Selected proteins      
    Glycoprotein Ib (platelet), alpha polypeptide NM_000173.1  3028.7, P   3630.25, P   0.28, NC   1.5  
    Glycoprotein VI (platelet)  AB043821.1   314.9, P   381.1, P   0.22, NC   NA  
    Glycoprotein IX (platelet) NM_000174.1  983.3, P   619.7, P   -0.54, NC   NA  
    Integrin, alpha 2b (platelet glycoprotein IIb of IIb/IIIa complex, antigen CD41B) NM_000419.2  3829.7, P   4136.4, P   0.33, NC   1.1  
    Integrin, beta 3 (platelet glycoprotein IIIa, antigen CD61)  AI189839   469.9, P   288.8, P   -0.38, NC   1.5  
    CD9 antigen (p24) NM_001769.1  2652.9, P   2360, P   -0.17, NC   NA  
Inhibitor      
    Transforming growth factor, beta 1 (Camurati-Engelmann disease)   BC000125.1   2953.9, P   3440.9, P   0.2, NC   1.1  
Growth factors      
    Interleukin 1, beta  M15330   208.4, P   154.6, P   -0.3, NC   NA  
    Interleukin 1, beta NM_000576.1  329.4, P   285, P   -0.27, NC   NA  
    Interleukin 6 (interferon, beta 2) NM_000600.1  —, A   16.9, P   -0.11, NC   NA  
    Interleukin 11 NM_000641.1  —, A   —, A   —, NC   NA  
    Platelet-derived growth factor alpha polypeptide NM_002607.1  223.9, P   267.5, P   0.4, I   1.5  
    Platelet-derived growth factor beta polypeptide  AK022920.1   21.7, P   23.7, P   -0.26, NC   NA  
    Platelet-derived growth factor C NM_016205.1  651.6, P   708.8, P   0.21, NC   1.4  
    Vascular endothelial growth factor  AF022375.1   568.6, P   467.2, P   -0.32, NC   NA  
Growth factors receptors      
    Interleukin 1 receptor NM_000877.1  —, A   —, A   —, NC   NA  
    Interleukin 3 receptor NM_002183.1  —, A   —, A   —, NC   NA  
    Interleukin 6 receptor NM_000565.1  67.1, P   43.2, P   -0.69, NC   0.1  
    Interleukin 11 receptor NM_004512.1  —, A   —, A   —, NC   NA  
    Myeloproliferative leukemia virus oncogene NM_005373.1  474.8, P   554.7, P   -0.01, NC   NA  
    V-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene homolog NM_000222.1  223.7, P   165.1, P   -0.9, D   NA  
    Chemokine (C-X-C motif) receptor 4  AJ224869   1505.5, P   957.7, P   -0.48, NC   1.0  
    Thromboxane A2 receptor  D38081   653.6, P   690.8, P   0.38, NC   1.5  
Cell cycle regulators      
    Cyclin B1  BE407516   128.6, P   159.5, P   0.15, NC   NA  
    Cyclin D3 NM_001760.1  3247.9, P   3241.9, P   0, NC   0.7  
    Cyclin E1  AI671049   31.1, P   —, A   -0.52, NC   NA  
    Cyclin E2 NM_004702.1  44.7, P   34, P   -0.24, NC   NA  
    Cell division cycle 2, G1 to S and G2 to M  AL524035   287, P   136.8, P   -0.65, D   NA  
    Cyclin-dependent kinase 2  M68520.1   240.9, P   172.4, P   -0.12, NC   NA  
    Cyclin-dependent kinase inhibitor 1B (p27, Kip1)  BC001971.1   309, P   232.5, P   -0.44, NC   NA  
    RAS p21 protein activator (GTPase activating protein) 1  M23612.1   339.9, P   271.2, P   -0.45, D   NA  
    Cyclin-dependent kinase inhibitor 2A (melanoma, p16, inhibits CDK4 NM_000077.1  —, A   —, A   —, NC   NA  
    RAS guanyl releasing protein 2 (calcium and DAG-regulated) NM_005825.1  637.9, P   868.2, P   0.36, NC   1.6  
Apoptosis regulators      
    BCL2-antagonist of cell death  U66879   37.6, P   23, A   -0.02, NC   NA  
    BCL2-antagonist/killer 1 NM_001188.1  66.2, P   82.1, P   -0.13, NC   NA  
    BCL2-like 2  D87461.1   112, P   174.2, P   0.42, NC   NA  
    B-cell CLL/lymphoma 2 NM_000633.1  16.6, P   21.1, P   0.3, NC   NA  
    Caspase-3, apoptosis-related cysteine protease NM_004346.1  115.3, P   155.4, P   0.53, I   1.7  
    Caspase-9, apoptosis-related cysteine protease  U60521.1   171.8, P   196.5, P   0.07, NC   0.9  
    Myeloid cell leukemia sequence 1 (BCL2-related) NM_021960.1  874.6, P   1078.7, P   0.3, NC   1.5  
Transcriptional regulators      
    Friend leukemia virus integration 1 NM_002017.2  538.1, P   322, P   -0.52, D   NA  
    GATA binding protein 1 (globin transcription factor 1)  M30601.1   606, P   677.6, P   0.18, NC   NA  
    GATA binding protein 2 NM_002050.1  67.7, P   83.2, P   -0.09, NC   NA  
    Jumonji homolog (mouse)  BG029530   1139.2, P   911.1, P   0.19, NC   NA  
    Jun D proto-oncogene NM_005354.2  2139.5, P   2371.8, P   0.24, NC   1.1  
    V-jun sarcoma virus 17 oncogene homolog (avian)  BE327172   8.45, P   27.6, P   1.26, NC   NA  
    Kruppel-like factor 1 (erythroid)  U65404.1   96, P   83, P   0.05, NC   NA  
    V-maf musculoaponeurotic fibrosarcoma oncogene homolog G (avian) NM_002359.1  299.5, P   286.9, P   0.39, NC   NA  
    V-myb myeloblastosis viral oncogene homolog (avian) NM_005375.1  85.2, P   96.35, P   -0.16, NC   NA  
    V-myc myelocytomatosis viral oncogene homolog (avian) NM_002467.1  317.6, P   353.6, P   0.42, NC   NA  
    V-fos FBJ murine osteosarcoma viral oncogene homolog  BC004490.1   —, A   —, A   —, NC   0.0  
    Nuclear factor (erythroid-derived 2), 45 kDa  L13974.1   1386.8, P   1419.3, P   0.03, NC   1.1  
    T-cell acute lymphocytic leukemia 1 NM_003189.1  1873.2, P   1763.8, P   0.03, NC   1.1  
    Ecotropic viral integration site 1  S82592.1   —, A   —, A   —, NC   NA  
    Core-binding factor, runt domain, alpha subunit 2; translocated to 3 NM_005187.2  234.7, P   377.1, P   0.57, I   1.6  
    Core-binding factor, runt domain, alpha subunit 2; translocated to 2 NM_005093.1  69.1, P   80.3, P   0.33, NC   NA  
Chemokines      
    GRO1 oncogene (melanoma growth stimulating activity, alpha) NM_001511.1  20.65, P   157.8, P   2.33, I   3.2  
    Stromal cell-derived factor 1  U19495.1   30.8, P   62.9, P   0.5, I   2.6  
    Interleukin 8 NM_000584.1  435.8, P   1601.3, P   1.87, I   3.9  
    Proplatelet basic protein  R64130   4476.1, P   4161.7, P   0.06, NC   0.7  
    Small inducible cytokine A5 (Rantes)  M21121   302.1, P   524.3, P   0.44, NC   NA  
    Platelet factor 4 NM_002619.1  6642.2, P   7639.8, P   0.12, NC   0.7  
Signal transducers      
    Signal transducer and activator of transcription  M97935   148.8, P   132, P   0.05, NC   NA  
    Signal transducer and activator of transcription 3  BC000627.1   503.3, P   375.2, P   -0.46, NC   NA  
    Signal transducer and activator of transcription 5A NM_003152.1  229.9, P   248.5, P   0.11, NC   NA  
    Mitogen-activated protein kinase 14 NM_001315.1  681, P   535.2, P   -0.57, D   0.9  
    Janus kinase 2 (a protein tyrosine kinase) NM_004972.2  45.5, P   34.6, P   -0.08, NC   NA  
    Phosphoinositide-3-kinase, class 2, beta polypeptide
 		NM_002646.1
 		 —, A
 		 —, A
 		 —, NC
 		 NA
 

P indicates present; NC, not changed; NA, not applicable; A, absent; I, increased; D, decreased; and —, absent gene showing aspecific signal.

View Large
Close Modal
This Feature Is Available To Subscribers Only

or Create an Account

Close Modal
Close Modal