Table 1.

Differential counts on Wright-Giemsa–stained cytospins from NBM, TBM, and TBM-BGI CD34+ cells from all 4 patients with thalassemia major after different days in culture showing the erythropoietic differentiation

Pronormoblast
Basophilic normoblast
Polychromatophilic normoblast
Orthochromic normoblast
RBC
WBC
NBM       
    d4   46 ± 5*  43 ± 5*  2 ± 0   0 ± 0   0 ± 0†  9 ± 1  
    d7   22 ± 4*  42 ± 4*  26 ± 4*  5 ± 4   1 ± 1†  5 ± 2  
    d10   12 ± 2   22 ± 5*  34 ± 3*  23 ± 9*  4 ± 1†  7 ± 2  
    d13   8 ± 1   15 ± 4   23 ± 3*  40 ± 5*  11 ± 2†  7 ± 2  
    d17   4 ± 2   10 ± 3   25 ± 3*  41 ± 4*  12 ± 3†  7 ± 1  
TBM       
    d4   48 ± 5*  39 ± 3*  5 ± 2   0 ± 0   0 ± 0   9 ± 1  
    d7   21 ± 3*  36 ± 2*  30 ± 3*  6 ± 4   1 ± 2†  5 ± 1  
    d10   11 ± 3   25 ± 5*  52 ± 5*  4 ± 2   1 ± 1†  9 ± 2  
    d13   8 ± 4   16 ± 6   49 ± 7*  11 ± 4   1 ± 1†  11 ± 4  
    d17   6 ± 3   16 ± 3   49 ± 7*  15 ± 4   3 ± 1†  12 ± 2  
TBM-BGI       
    d4   49 ± 6*  36 ± 8*  3 ± 1   0 ± 0   0 ± 0   13 ± 2  
    d7   16 ± 6*  36 ± 8*  28 ± 3*  11 ± 8   1 ± 1†  10 ± 3  
    d10   8 ± 4   20 ± 7   33 ± 5*  28 ± 11*  5 ± 2†  7 ± 2  
    d13   7 ± 3   14 ± 1   30 ± 5*  34 ± 8*  10 ± 3†  6 ± 2  
    d17
 		 5 ± 2
 		 13 ± 3
 		 21 ± 7
 		 35 ± 5*
 		 14 ± 3†
 		 13 ± 4
 
Pronormoblast
Basophilic normoblast
Polychromatophilic normoblast
Orthochromic normoblast
RBC
WBC
NBM       
    d4   46 ± 5*  43 ± 5*  2 ± 0   0 ± 0   0 ± 0†  9 ± 1  
    d7   22 ± 4*  42 ± 4*  26 ± 4*  5 ± 4   1 ± 1†  5 ± 2  
    d10   12 ± 2   22 ± 5*  34 ± 3*  23 ± 9*  4 ± 1†  7 ± 2  
    d13   8 ± 1   15 ± 4   23 ± 3*  40 ± 5*  11 ± 2†  7 ± 2  
    d17   4 ± 2   10 ± 3   25 ± 3*  41 ± 4*  12 ± 3†  7 ± 1  
TBM       
    d4   48 ± 5*  39 ± 3*  5 ± 2   0 ± 0   0 ± 0   9 ± 1  
    d7   21 ± 3*  36 ± 2*  30 ± 3*  6 ± 4   1 ± 2†  5 ± 1  
    d10   11 ± 3   25 ± 5*  52 ± 5*  4 ± 2   1 ± 1†  9 ± 2  
    d13   8 ± 4   16 ± 6   49 ± 7*  11 ± 4   1 ± 1†  11 ± 4  
    d17   6 ± 3   16 ± 3   49 ± 7*  15 ± 4   3 ± 1†  12 ± 2  
TBM-BGI       
    d4   49 ± 6*  36 ± 8*  3 ± 1   0 ± 0   0 ± 0   13 ± 2  
    d7   16 ± 6*  36 ± 8*  28 ± 3*  11 ± 8   1 ± 1†  10 ± 3  
    d10   8 ± 4   20 ± 7   33 ± 5*  28 ± 11*  5 ± 2†  7 ± 2  
    d13   7 ± 3   14 ± 1   30 ± 5*  34 ± 8*  10 ± 3†  6 ± 2  
    d17
 		 5 ± 2
 		 13 ± 3
 		 21 ± 7
 		 35 ± 5*
 		 14 ± 3†
 		 13 ± 4
 

Data are represented as mean ± SEM from 200 cells counted from 4 different TBM patients and normal controls; P < .01 (NBM vs TBM), p = NS (NBM vs TBM-BGI).

RBCs indicate red blood cells; WBC, white blood cells.

*

The predominant stages of maturation on any particular day in culture.

†

Mature RBCs.

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