Quantitative analysis of EGFP+ cells in infarcted area
. | EGFP+ cells . | . | |
|---|---|---|---|
| Mice . | Total* . | Actinin+ . | |
| w-BM G-CSF (-) | 8841 | 65 | |
| (n = 5) | (1478, 1757, 1515, 2348, 1743) | (18, 15, 9, 9, 14) | |
| w-BM G-CSF (+) | 119802 | 5403 | |
| (n = 5) | (25006, 19950, 28303, 17633, 28910) | (1016, 982, 1328, 921, 1156) | |
| CD34- KSL-SP G-CSF (-) | 1224 | 0 | |
| (n = 5) | (254, 183, 210, 300, 277) | (0, 0, 0, 0, 0) | |
| CD34- KSL-SP G-CSF (+) | 41779 | 3 | |
| (n = 5) | (8654, 7005, 9217, 9034, 7869) | (0, 0, 1, 0, 2) | |
. | EGFP+ cells . | . | |
|---|---|---|---|
| Mice . | Total* . | Actinin+ . | |
| w-BM G-CSF (-) | 8841 | 65 | |
| (n = 5) | (1478, 1757, 1515, 2348, 1743) | (18, 15, 9, 9, 14) | |
| w-BM G-CSF (+) | 119802 | 5403 | |
| (n = 5) | (25006, 19950, 28303, 17633, 28910) | (1016, 982, 1328, 921, 1156) | |
| CD34- KSL-SP G-CSF (-) | 1224 | 0 | |
| (n = 5) | (254, 183, 210, 300, 277) | (0, 0, 0, 0, 0) | |
| CD34- KSL-SP G-CSF (+) | 41779 | 3 | |
| (n = 5) | (8654, 7005, 9217, 9034, 7869) | (0, 0, 1, 0, 2) | |
Whole bone marrow cells (w-BM) or single CD34- c-kit+Sca-1+ lineage- side population (CD34- KSL-SP) cells from the BM of EGFP mice were transplanted into lethally irradiated C57BL/6 mice. Myocardial infarction (MI) was induced 8 weeks later. Commencing 24 hours after induction of MI, a saline solution (G-CSF [-]) or G-CSF (G-CSF [+]) was injected into the mice for 10 consecutive days. Eight weeks after induction of MI, the mice were sacrificed, and the hearts were subjected to immunohistological analysis. The number of EGFP+ and EGFP+ actinin+ cells in the infarcted area was scored using 100 specimens per group of mice (20 samples from the mid-left ventricle per mouse).
Total numbers of EGFP+ cells in the infarcted area.