Numbers of immunogold particles for VWF and P-selection in the granules and in the cytoplasm of MKs from the spleen and in platelets purified from the blood of normal and GATA-1low mice
. | VWF . | . | . | P-selectin . | . | . | ||||
|---|---|---|---|---|---|---|---|---|---|---|
. | Granules . | Cytoplasm/DMS . | Background . | Granules . | Cytoplasm/DMS . | Background . | ||||
| Wild-type | ||||||||||
| Stage I MK (32 μm) | BD | < 1 | ND | BD | < 1 | ND | ||||
| Stage II MK (50 μm) | 79.8 ± 5.5 | 284.8 ± 40.0 | 2.8 ± 1.9 | 240.2 ± 30.5* | 331.6 ± 42.3 | 20.8 ± 6.5 | ||||
| Stage III MK (75 μm) | 82.0 ± 5.8 | 56.8 ± 5.8* | 3.2 ± 2.3 | 427.0 ± 11.2 | 148.0 ± 7.6* | 18.4 ± 9.4 | ||||
| Platelet | 5.8 ± 0.8 | 2.2 ± 1.3 | < 1 | 8.0 ± 4 | 3.6 ± 2.5 | < 1 | ||||
| GATA-1low | ||||||||||
| Light MK | 16.4 ± 3.8* | 57.0 ± 7.2* | 3.2 ± 2.3 | 188.0 ± 21.7* | 200.0 ± 50.5* | 8.0 ± 2.5 | ||||
| Heavy MK | 5.0 ± 1.9* | 61.8 ± 4.5* | 0.8 ± 0.8 | 144.0 ± 78.3* | 466.0 ± 174.6* | 10.8 ± 6.9 | ||||
| Platelet | 0.4 ± 0.5† | 1.2 ± 1.8 | < 1 | 0.4 ± 0.9† | 0.8 ± 0.8† | < 1 | ||||
. | VWF . | . | . | P-selectin . | . | . | ||||
|---|---|---|---|---|---|---|---|---|---|---|
. | Granules . | Cytoplasm/DMS . | Background . | Granules . | Cytoplasm/DMS . | Background . | ||||
| Wild-type | ||||||||||
| Stage I MK (32 μm) | BD | < 1 | ND | BD | < 1 | ND | ||||
| Stage II MK (50 μm) | 79.8 ± 5.5 | 284.8 ± 40.0 | 2.8 ± 1.9 | 240.2 ± 30.5* | 331.6 ± 42.3 | 20.8 ± 6.5 | ||||
| Stage III MK (75 μm) | 82.0 ± 5.8 | 56.8 ± 5.8* | 3.2 ± 2.3 | 427.0 ± 11.2 | 148.0 ± 7.6* | 18.4 ± 9.4 | ||||
| Platelet | 5.8 ± 0.8 | 2.2 ± 1.3 | < 1 | 8.0 ± 4 | 3.6 ± 2.5 | < 1 | ||||
| GATA-1low | ||||||||||
| Light MK | 16.4 ± 3.8* | 57.0 ± 7.2* | 3.2 ± 2.3 | 188.0 ± 21.7* | 200.0 ± 50.5* | 8.0 ± 2.5 | ||||
| Heavy MK | 5.0 ± 1.9* | 61.8 ± 4.5* | 0.8 ± 0.8 | 144.0 ± 78.3* | 466.0 ± 174.6* | 10.8 ± 6.9 | ||||
| Platelet | 0.4 ± 0.5† | 1.2 ± 1.8 | < 1 | 0.4 ± 0.9† | 0.8 ± 0.8† | < 1 | ||||
Mice were 4 to 6 months of age. At least 15 to 20 wild-type and more than 50 GATA-1low MKs were analyzed for the expression of VWF and P-selectin. The numbers of gold particles present in the different regions (14 μm2 of MKs or in the entire platelet) are presented as mean ± SD of determinations obtained in 5 randomly selected cells for experimental point. The number of gold granules present in 5 randomly selected 14-μm2 surfaces outside the cells is also presented as control (background).
BD indicates granules not detectable; ND, not done.
Indicates data statistically different (P < .05) from those observed in normal stage II MKs. and in
Indicate data statistically different (P < .05) from those observed in platelets.