Donor CD8+ T-cell apoptosis
. | Annexin, % . |
---|---|
Syn + IL-18 | 26.8 ± 11.7 |
Allo + control | 47.4 ± 18.1 |
Allo + IL-18 | 48.0 ± 16.6 |
. | Annexin, % . |
---|---|
Syn + IL-18 | 26.8 ± 11.7 |
Allo + control | 47.4 ± 18.1 |
Allo + IL-18 | 48.0 ± 16.6 |
After irradiation, bm 1 mice underwent transplantation from B6. Ly-5a (CD45.1+) donors and were treated with IL-18 or the diluent, as described in “Materials and methods.” Splenocytes were pooled from 3 mice per group and were analyzed on day 5 after BMT by 3-color flow cytometry for expression of annexin, CD45.1+, and CD8+ cells. Gates were set for CD45.1+CD8+ (donor) cells, and the percentage of cells expressing annexin was determined. Data are from 1 of 2 similar experiments and represent mean ± SE. P = .84, allogeneic plus IL-18 vs allogeneic plus PBS.