Table 1. Differential cytokine... | American Society of Hematology

Table 1.

Differential cytokine production by Lewis CD45RC^high and CD45RC^low CD8 T cells

Experiment and CD8 T-cell subpopulation	Proliferation, CPM	IL-2, pg/mL	IFNγ, U/mL	IL-10, pg/mL	IL-4, AU	IL-13, AU
1
CD45RC^high	29 031	2539	226	UND	34	UND
CD45RC^low	30 975	1931	279	495	1034	986
2
CD45RC^high	37 316	6779	617	UND	UND	UND
CD45RC^low	36 358	1986	656	787	1289	UND
3
CD45RC^high	ND	UND	453	UND	UND	UND
CD45RC^low	ND	UND	296	282	119	28
4
CD45RC^high	21 209	1070	336	UND	44	8
CD45RC^low	16 469	UND	98	603	372	77

Experiment and CD8 T-cell subpopulation	Proliferation, CPM	IL-2, pg/mL	IFNγ, U/mL	IL-10, pg/mL	IL-4, AU	IL-13, AU
1
CD45RC^high	29 031	2539	226	UND	34	UND
CD45RC^low	30 975	1931	279	495	1034	986
2
CD45RC^high	37 316	6779	617	UND	UND	UND
CD45RC^low	36 358	1986	656	787	1289	UND
3
CD45RC^high	ND	UND	453	UND	UND	UND
CD45RC^low	ND	UND	296	282	119	28
4
CD45RC^high	21 209	1070	336	UND	44	8
CD45RC^low	16 469	UND	98	603	372	77

Tissue culture supernatants were collected 24 and 48 hours after stimulation with anti-TCR and anti-CD28 and were assayed for IL-2, IL-10, and IFN-γ using capture ELISA as described in “Materials and methods.” The results shown concern IL-2 production after 24 hours of stimulation and IL-10 or IFNγ production after 48 hours of stimulation. IL-4 and IL-13 mRNA were detected by RT-PCR after stimulation for 24 hours.

ND indicates not determined; UND, undetectable.

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